Factors influencing the infant gut microbiome at age 3-6 months: Findings from the ethnically diverse Vitamin D Antenatal Asthma Reduction Trial (VDAART).
Factors influencing the infant gut microbiome at age 3-6 months: Findings from the ethnically diverse Vitamin D Antenatal Asthma Reduction Trial (VDAART).
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DOI：
10.1016/j.jaci.2016.08.045
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发表时间：
2017-02
期刊：
影响因子：
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通讯作者：
Litonjua AA
中科院分区：
文献类型：
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作者：
Sordillo JE;Zhou Y;McGeachie MJ;Ziniti J;Lange N;Laranjo N;Savage JR;Carey V;O'Connor G;Sandel M;Strunk R;Bacharier L;Zeiger R;Weiss ST;Weinstock G;Gold DR;Litonjua AA
婴儿期的肠道微生物组影响免疫系统的成熟，并可能对过敏性疾病风险产生重要影响。确定产前和早期生活因素如何影响3-6个月龄婴儿的相对较大的种族多样性研究人群的肠道微生物组，这些婴儿参加了VDAART，这是一项妊娠期补充维生素D以预防后代哮喘和过敏的临床试验。我们对333名婴儿的粪便样本进行了16 S rRNA基因测序。微生物多样性的计算采用香农指数。因子分析应用于前25个最丰富的类群揭示了四个潜在的细菌共丰度组;第一个占主导地位的厚壁菌门（毛螺菌科/梭菌目），第二个变形菌门（克雷伯氏菌/肠杆菌），第三个拟杆菌，第四个韦荣氏球菌。共丰度组的分数被用作回归模型的结果，产前/出生和人口统计学特征作为独立的预测因子。还使用所有微生物群落成员进行了多变量分析。高加索人种/种族与较低的多样性，但较高的拟杆菌共丰度得分。与非裔美国人相比，白人婴儿的变形菌门评分较低。剖腹产与较高的多样性，但减少拟杆菌共同丰度分数。厚壁菌门和变形菌门的得分较高的婴儿出生的剖腹产。母乳喂养的婴儿有较低的比例梭菌。脐带血维生素D与毛杆菌增加有关，但与乳球菌减少有关。研究结果表明，种族、分娩方式、母乳喂养和脐带血维生素D水平与婴儿肠道微生物组组成有关，可能对免疫系统调节和哮喘/过敏性疾病发病率产生长期影响。这项研究的结果表明，种族，分娩方式，母乳喂养和脐带血维生素D水平与婴儿肠道微生物组组成有关，对免疫系统调节和哮喘/过敏性疾病的发病率具有潜在的长期影响。
The gut microbiome in infancy influences immune system maturation, and may have an important impact allergic disease risk. To determine how prenatal and early life factors impact the gut microbiome in a relatively large, ethnically diverse study population of infants at 3–6 months of age, who were enrolled in VDAART, a clinical trial of vitamin D supplementation in pregnancy to prevent asthma and allergies in offspring. We performed 16S rRNA gene sequencing on 333 infants’ stool samples. Microbial diversity was computed using the Shannon Index. Factor analysis applied to the top 25 most abundant taxa revealed four underlying bacterial co-abundance groups; the first dominated by Firmicutes (Lachnospiraceae/ Clostridiales), the second by Proteobacteria (Klebsiella/Enterobacter), the third by Bacteriodetes, and the fourth by Veillonella. Scores for co-abundance groups were used as outcomes in regression models, with prenatal/birth and demographic characteristics as independent predictors. Multivariate analysis, using all microbial community members, was also conducted. Caucasian race/ethnicity was associated with lower diversity but higher Bacteroidetes co-abundance scores. Caucasian infants had lower Proteobacteria scores as compared to African Americans. C-section birth was associated with higher diversity, but with decreased Bacteroidetes co-abundance scores. Firmicutes and Proteobacteria scores were higher for infants born by C-section. Breastfed infants had lower proportions of Clostridiales. Cord blood vitamin D was linked to increased Lachnobacterium, but decreased Lactococcus. The findings presented here suggest that race, mode of delivery, breastfeeding and cord blood vitamin D levels are associated with infant gut microbiome composition, with possible long-term implications for immune system modulation and asthma/allergic disease incidence. The findings presented in this study suggest that race, mode of delivery, breastfeeding and cord blood vitamin D levels are associated with infant gut microbiome composition, with potential long-term implications for immune system modulation and asthma/allergic disease incidence.