Cathelicidin antimicrobial peptide LL-37 in psoriasis enables keratinocyte reactivity against TLR9 ligands.
Cathelicidin antimicrobial peptide LL-37 in psoriasis enables keratinocyte reactivity against TLR9 ligands.
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DOI：
10.1038/jid.2011.259
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发表时间：
2012-01
影响因子：
6.5
通讯作者：
Gallo, Richard L.
中科院分区：
文献类型：
--
作者：
Morizane, Shin;Yamasaki, Kenshi;Muehleisen, Beda;Kotol, Paul F.;Murakami, Masamoto;Aoyama, Yumi;Iwatsuki, Keiji;Hata, Tissa;Gallo, Richard L.
在这里，我们表明，在银屑病皮损皮肤角质形成细胞表达增加Toll样受体（TLR）9，类似地定位与表达升高的抗菌肽LL-37。在培养中，暴露于LL-37的正常人角质形成细胞增加TLR9表达。此外，当角质形成细胞暴露于LL-37并随后用TLR9配体如CpG或基因组DNA处理时，角质形成细胞大大增加了I型干扰素的产生。这种反应模拟了银肩病皮损表皮中的观察结果，因为银肩病皮损中的角质形成细胞比缺乏LL-37的正常皮肤产生更大量的干扰素-β。角质形成细胞中I型干扰素的诱导机制依赖于TLR9的表达，但不依赖于DNA-LL-37复合物。这些发现表明，角质形成细胞识别和响应DNA，并能积极参与有助于银屑病的免疫环境。
Here we show that keratinocytes in psoriatic lesional skin express increased Toll-like receptor (TLR) 9 that similarly localizes with elevated expression of the cathelicidin antimicrobial peptide LL-37. In culture, normal human keratinocytes exposed to LL-37 increased TLR9 expression. Furthermore, when keratinocytes were exposed to LL-37 and subsequently treated with TLR9 ligands such as CpG or genomic DNA, keratinocytes greatly increased production of type I interferons. This response mimicked observations in the epidermis of psoriatic lesional skin as keratinocytes in psoriatic lesions produce greater amounts of interferon-β than normal skin lacking LL-37. The mechanism for induction of type I interferons in keratinocytes was dependent on TLR9 expression but not on a DNA-LL-37 complex. These findings suggest that keratinocytes recognize and respond to DNA and can actively participate in contributing to the immunological environment that characterizes psoriasis.
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DOI：
10.1073/pnas.91.23.11035
发表时间：
1994-11-08
影响因子：
11.1
作者：
GALLO, RL;ONO, M;BERNFIELD, M
通讯作者：
BERNFIELD, M
影响因子：
17.1
作者：
Dombrowski Y;Peric M;Koglin S;Kammerbauer C;Göss C;Anz D;Simanski M;Gläser R;Harder J;Hornung V;Gallo RL;Ruzicka T;Besch R;Schauber J
通讯作者：
Schauber J
影响因子：
15.3
作者：
Nestle, Frank O;Conrad, Curdin;Tun-Kyi, Adrian;Homey, Bernhard;Gombert, Michael;Boyman, Onur;Burg, Gunter;Liu, Yong-Jun;Gilliet, Michel
通讯作者：
Gilliet, Michel
DOI：
10.1038/jid.2009.435
发表时间：
2010-05
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.8
作者：
Frohm, M;Agerberth, B;Gudmundsson, GH
通讯作者：
Gudmundsson, GH