Bacteria in the apical root canal of teeth with primary apical periodontitis
Bacteria in the apical root canal of teeth with primary apical periodontitis
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DOI：
10.1016/j.tripleo.2009.01.042
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发表时间：
2009-05-01
期刊：
影响因子：
--
通讯作者：
Silva, Marlei G.
中科院分区：
文献类型：
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作者：
Siqueira, Jose F., Jr.;Rocas, Isabela N.;Silva, Marlei G.
目标。根尖根管中的细菌对根周组织有一定的损伤作用。因此，在该区域发现的物种可能与根尖周炎的发病机制特别相关。本研究调查了几种细菌在根尖周炎患牙根管中的出现和水平。研究设计。从表现为慢性根尖周炎病变的拔除牙齿的根管根尖部分提取DNA作为模板，采用基于16S核糖体RNA基因的反向捕获棋盘杂交方法分析28种细菌/系统类型的存在和水平。20份标本中有19份检出细菌DNA。检出的分类群包括溶乳假单胞菌(32%)、类杆菌克隆体X083(26%)、链球菌(21%)、乌尔森氏菌(10.5%)、增效菌克隆BA121(10.5%)、核梭杆菌(10.5%)、齿内卟啉单胞菌(10.5%)、辩证菌克隆BS016(5%)、纤毛因子(5%)、微小副单胞菌(5%)和齿密螺旋体(5%)。其中只有拟杆菌克隆X083和协生杆菌克隆BA121在10(5)以上。这些细菌在感染根管的根尖部分的出现表明它们在根尖周炎的病因学中可能起到致病作用。《口腔外科》《口腔医学》《口腔病理学》《口腔放射学》2009；107：721-726)
Objective. Bacteria settled in the apical root canal are in a privileged position to inflict damage to the periradicular tissues. Therefore, the species identified in this region can be of special relevance for the pathogenesis of apical periodontitis. This study investigated the occurrence and levels of several bacterial taxa in the apical root canal of teeth with apical periodontitis.Study design. DNA extracts from samples taken from the apical part of the root canal of extracted teeth evincing chronic apical periodontitis lesions served as templates for analysis of the presence and levels of 28 bacterial species/ phylotypes using a 16S ribosomal RNA gene-based reverse-capture checkerboard hybridization assay.Results. Bacterial DNA was detected in 19 out of 20 samples. Detected taxa included Pseudoramibacter alactolyticus (32%), Bacteroidetes clone X083 (26%), Streptococcus species (21%), Olsenella uli (10.5%), Synergistes clone BA121 (10.5%), Fusobacterium nucleatum (10.5%), Porphyromonas endodontalis (10.5%), Dialister clone BS016 (5%), Filifactor alocis (5%), Parvimonas micra (5%), and Treponema denticola (5%). Of these, only Bacteroidetes clone X083 and Synergistes clone BA121 were found at levels above 10(5).Conclusion. Occurrence of these bacterial taxa in the apical part of infected root canals indicates their potential pathogenetic role in the etiology of apical periodontitis. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009; 107:721-726)