Molecular characterization of two strains of Shope fibroma virus.
Molecular characterization of two strains of Shope fibroma virus.
复制标题
肖普纤维瘤病毒两种毒株的分子特征。
DOI：
10.1016/0042-6822(85)90354-x
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发表时间：
1985
期刊：
影响因子：
3.7
通讯作者：
Pogo,BG
中科院分区：
文献类型：
--
作者：
Berkowitz,EM;Pogo,BG
一株分离的Shope纤维瘤病毒（SFV），命名为Indiana (SFV- i)，先前被描述为体内SFV的致瘤剂，体外细胞毒素是正痘病毒（牛痘、兔痘等）的致瘤剂，并且与SFV和牛痘具有相同的抗原决定因子。采用Southern印迹和杂交技术研究了SFV- 1与SFV和牛痘的遗传亲缘关系。结果表明，SFV- 1与牛痘病毒具有广泛的同源性，但很少有共同的序列。相比之下，SFV与牛痘没有序列同源性。这些结果表明，SFV-I是一种正痘病毒，它携带了一些来自麻痘病毒的遗传信息。两属痘病毒之间的重组以前没有报道过。
An isolate of Shope fibroma virus (SFV), designated Indiana (SFV-I), was previously described to be tumorigenicin vivoas SFV, cytocidalin vitroas the orthopoxviruses (vaccinia, rabbitpox, etc.), and to share antigenic determinants with SFV and vaccinia. The genetic relatedness of SFV-I to SFV and vaccinia was studied by means of Southern blotting and hybridization. The results indicated that SFV-I shares extensive DNA homology with vaccinia, but few common sequences with SFV. By contrast, SFV and vaccinia show no sequence homology. These findings suggest that SFV-I is an orthopoxvirus which carries some genetic information from the leporipoxviruses. Recombinants between two genera of poxviruses have not been reported before.