Statistical optimization of medium components to improve the chitinase activity of Streptomyces sp Da11 associated with the South China Sea sponge Craniella australiensis
Statistical optimization of medium components to improve the chitinase activity of Streptomyces sp Da11 associated with the South China Sea sponge Craniella australiensis
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DOI：
10.1016/j.procbio.2008.05.014
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发表时间：
2008-10-01
影响因子：
4.4
通讯作者：
Zhang, Fengli
中科院分区：
文献类型：
--
作者：
Han, Yue;Li, Zhiyong;Zhang, Fengli
本文应用统计Plackett-Burman设计和Box-Behnken响应面方法对链霉菌几丁质酶活力的培养基组分进行优化。DA11与南中国海绵澳大利亚颅骨藻有关。首先，通过单因素系列析因试验确定了半乳糖和蛋白胨是该菌生长和几丁质酶活力的适宜碳源和氮源。其次，半乳糖、胶体甲壳素和硫酸镁中心点7H(2)O对几丁质酶活性有显著影响。通过Box-Behnken法得到的最适培养基组成为：5.00g/L半乳糖，2.62g/L胶体甲壳素，0.10g/L硫酸镁中心点7H(2)O和12.5g/L蛋白胨，1.5g/L PO43-(KH_2PO_4 0.45g/L，K_2HPO_4 1.05g/L)，12.5g/L粉甲壳素，0.0-3g/L FeSO_4和0.03g/L锌中心点7H(2)O加人工海水(ASW)。在此优化条件下，几丁质酶活性和细胞生长均得到显著提高。发酵7h后，几丁质酶活力达到1559.2 U/g细胞干重(36.43U/mL)，最大细胞干重23.3g/L，分别是基础培养基数的39.2倍和2.6倍。结果表明，链霉菌属(Streptomyces sp.)从海绵中分离到的DA11菌株可能是一株产几丁质酶的潜在菌株。据我们所知，这是海绵伴生链霉菌生产几丁质酶的统计优化的第一份报告。(C)2008爱思唯尔有限公司。保留所有权利。
In this paper, statistical Plackett-Burman design and Box-Behnken Response Surface Methodology were applied to optimize the medium components to improve the chitinase activity of Streptomyces sp. DA11 associated with South China Sea sponge Craniella australiensis. Firstly, galactose and peptone were found to be the suitable carbon and nitrogen sources for the growth and chitinase activity by single factor Seriatim-Factorial test. Secondly, galactose, colloidal chitin and MgSO4 center dot 7H(2)O were proved to have remarkable effects on chitinase activity. Finally, an optimal medium was obtained by Box-Behnken methodology, which consisted of 5.00 g/L galactose, 2.62 g/L colloidal chitin, 0.10 g/L MgSO4 center dot 7H(2)O and 12.5 g/L peptone, 1.5 g/L PO43- (KH2PO4 0.45 g/L, K2HPO4 1.05 g/L), 12.5 g/L powder chitin, 0.0-3 g/L FeSO4 and 0.03 g/L ZnSO4 center dot 7H(2)O with artificial sea water (ASW). With this optimal medium, both the chitinase activity and cell growth were remarkably enhanced. The chitinase activity of 1559.2 U/g cell dry weight (36.43 U/mL) and the maximum cell dry weight of 23.3 g/L were reached after incubation of 7 2 h, which were 39.2-fold and 2.6-fold higher than that of the basic medium, respectively. The results suggest that Streptomyces sp. DA11 isolated from marine sponge might be a potential strain for the production of chitinolytic enzymes. To our knowledge, this is the first report of the statistical optimization of chitinase production from marine sponge-associated Streptomyces. (C) 2008 Elsevier Ltd. All rights reserved.