Human uterine lymphocytes acquire a more experienced and tolerogenic phenotype during pregnancy.
Human uterine lymphocytes acquire a more experienced and tolerogenic phenotype during pregnancy.
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DOI：
10.1038/s41598-017-03191-0
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发表时间：
2017-06-06
影响因子：
4.6
通讯作者：
van der Molen RG
中科院分区：
文献类型：
--
作者：
Feyaerts D;Benner M;van Cranenbroek B;van der Heijden OWH;Joosten I;van der Molen RG
妊娠需要微妙的免疫平衡，以养育同种异体胎儿，同时保持对病原体的反应性。尽管越来越多的知识，数据是缺乏对妊娠前子宫内膜淋巴细胞的过渡到妊娠状态。在这里，我们免疫表型淋巴细胞从子宫内膜（MMC），长期壁蜕膜（DPMC），和PBMC直接比较。我们发现MMC和DPMC的免疫细胞组成明显不同，在DPMC中NK细胞较少，而NKT细胞和T细胞较多。中央记忆和效应记忆T细胞的百分比增加，以及DPMC中幼稚T细胞的减少表明蜕膜T细胞比子宫内膜T细胞更有经验。DPMC中CD 4 + CD 25 highCD 127 − Treg的百分比增加，包括分化的Treg，表明妊娠期间经历了更丰富的耐受性环境。MMC和DPMC的Th细胞组成与PBMC不同，在子宫环境中Th 1优于Th 2。MMC和DPMC之间Th细胞亚群的百分比没有显著差异。我们的研究结果表明，在怀孕之前，Th 1/Th 2/Th 17平衡已经受到严格控制。这些发现创造了机会，以进一步研究潜在的免疫机制，妊娠并发症使用经血作为子宫内膜淋巴细胞的来源。
Pregnancy requires a delicate immune balance that nurtures the allogeneic fetus, while maintaining reactivity against pathogens. Despite increasing knowledge, data is lacking on the transition of pre-pregnancy endometrial lymphocytes to a pregnancy state. Here, we immunophenotyped lymphocytes from endometrium (MMC), term decidua parietalis (DPMC), and PBMC for direct comparison. We found that the immune cell composition of MMC and DPMC clearly differ from each other, with less NK-cells, and more NKT-cells and T-cells in DPMC. An increased percentage of central memory and effector memory T-cells, and less naive T-cells in DPMC indicates that decidual T-cells are more experienced than endometrial T-cells. The increased percentage of CD4+CD25highCD127− Treg in DPMC, including differentiated Treg, is indicative of a more experienced and tolerogenic environment during pregnancy. The Th cell composition of both MMC and DPMC was different from PBMC, with a preference for Th1 over Th2 in the uterine environment. Between MMC and DPMC, percentages of Th cell subsets did not differ significantly. Our results suggest that already before pregnancy a tightly controlled Th1/Th2/Th17 balance is present. These findings create opportunities to further investigate the underlying immune mechanism of pregnancy complications using menstrual blood as a source for endometrial lymphocytes.