Transcriptome analysis of encystation in Entamoeba invadens.
Transcriptome analysis of encystation in Entamoeba invadens.
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DOI：
10.1371/journal.pone.0074840
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Nozaki T
中科院分区：
文献类型：
--
作者：
De Cádiz AE;Jeelani G;Nakada-Tsukui K;Caler E;Nozaki T
包囊形成是一组肠道原生动物（包括溶组织内阿米巴，肠道和肠外阿米巴病的病原体）完成生命周期的重要分化过程。然而，在成囊过程中的基因表达调控知之甚少。为了在分子水平上全面了解这一过程， E . invadens，这是一个相关的爬行动物物种，导致类似于E.在包囊形成过程中研究了溶组织菌。使用定制生成的Affyphase平台微阵列，我们进行了包囊形成的时程（0.5、2、8、24、48和120 h）基因表达分析。 E .入侵。ANOVA分析显示，相对于滋养体阶段，总共1，528个基因在一个或多个时间点显示出≥3倍的上调。在这些被调控的基因中，8%（116个基因）在早期时间点（0.5、2和8h）上调，而63%（962个基因）在后期时间点（24、48和120h）上调。29%（450个基因）在2至5个时间点上调或在早期和晚期均组成性上调。上调的基因包括编码转运蛋白、细胞骨架蛋白、参与囊泡运输的蛋白（小GTP酶）、Myb转录因子、半胱氨酸蛋白酶、蛋白酶体组分和几丁质生物合成酶的基因。本研究首次对内阿米巴从侵入性滋养体到休眠感染性包囊阶段的分化过程中基因表达进行了动态分析。对单个基因及其编码产物进行功能分析，可能会发现新的化疗药物的发展目标，干扰寄生虫的阶段转换。
Encystation is an essential differentiation process for the completion of the life cycle of a group of intestinal protozoa including Entamoeba histolytica, the causative agent of intestinal and extraintestinal amebiasis. However, regulation of gene expression during encystation is poorly understood. To comprehensively understand the process at the molecular level, the transcriptomic profiles of E . invadens , which is a related reptilian species that causes an invasive disease similar to that of E. histolytica, was investigated during encystation. Using a custom-generated Affymetrix platform microarray, we performed time course (0.5, 2, 8, 24, 48, and 120 h) gene expression analysis of encysting E . invadens . ANOVA analysis revealed that a total of 1,528 genes showed ≥3 fold up-regulation at one or more time points, relative to the trophozoite stage. Of these modulated genes, 8% (116 genes) were up-regulated at the early time points (0.5, 2 and 8h), while 63% (962 genes) were up-regulated at the later time points (24, 48, and 120 h). Twenty nine percent (450 genes) are either up-regulated at 2 to 5 time points or constitutively up-regulated in both early and late stages. Among the up-regulated genes are the genes encoding transporters, cytoskeletal proteins, proteins involved in vesicular trafficking (small GTPases), Myb transcription factors, cysteine proteases, components of the proteasome, and enzymes for chitin biosynthesis. This study represents the first kinetic analysis of gene expression during differentiation from the invasive trophozoite to the dormant, infective cyst stage in Entamoeba . Functional analysis on individual genes and their encoded products that are modulated during encystation may lead to the discovery of targets for the development of new chemotherapeutics that interfere with stage conversion of the parasite.
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