Genotypic and Phenotypic Cross-Drug Resistance of Harboring Drug-Resistant HIV Type 1 Subtype B′ Strains from Former Blood Donors in Central Chinese Provinces
Genotypic and Phenotypic Cross-Drug Resistance of Harboring Drug-Resistant HIV Type 1 Subtype B′ Strains from Former Blood Donors in Central Chinese Provinces
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DOI：
10.1089/aid.2009.0252
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发表时间：
2010-09-01
影响因子：
1.5
通讯作者：
Shao, Yiming
中科院分区：
文献类型：
--
作者：
Ma, Liying;Huang, Jianghong;Shao, Yiming
本研究的目的是评估来自中国中部地区的HIV-1 B'亚型（Thai B'，HIV-1 B的一个分支）献血者感染人群的基因型和表型耐药模式，目的是设计有效的治疗方案。对65例患者的HIV-1 pol基因进行了测序和耐药性评估，同时从患者中分离的病毒用于基于TZM-bl细胞系的表型分析。所有携带HIV-1 RTIs耐药突变的HIV-1毒株对EFV（100%）和DLV（92%）、ABC（84%）和TDF（77%）的交叉耐药率均高于FTC和3 TC（42%）。AZT/ddI/NVP组胸苷类似物突变（TAM）相关突变（62.5%）多于d4 T/ddI/NVP组（32.65%）。表型测定显示基因型和表型交叉抗性之间高度一致。本研究表明，中国艾滋病患者中存在高水平的HIV-1 RTIs交叉耐药，并且对3 TC的原发耐药率也很高，这表明由于NRTI和NNRTI突变的高流行率，应该在所有初治患者中实现基因型。
The objective of this study was to assess the patterns of genotypic and phenotypic resistance in a population of blood donor patients infected with HIV-1 subtype B' (Thai B', a clade of HIV-1 B) from central China, previously treated and harboring NRTI and NNRTI resistance mutations, with the purpose of designing effective therapeutic regimens. The HIV-1 pol genes from 65 patients were sequenced and estimated for drug resistance while the viruses isolated from the patients were used to analyze the phenotype based on the TZM-bl cell line. All the HIV-1 strains harboring one or more drug resistance mutations to HIV-1 RTIs possessed high cross-resistance to EFV (100%) and DLV (92%), as well as to ABC (84%) and TDF (77%), which are much higher than both FTC and 3TC (42%). There were more thymidine analog mutation (TAM)-associated mutations in the AZT/ddI/NVP group (62.5%) than in the d4T/ddI/NVP group (32.65%). A phenotypic assay showed high concordance between genotypic and phenotypic cross-resistance. This study showed there was a high level of cross-drug resistance to HIV-1 RTIs among Chinese AIDS patients harboring resistant strains, and there is also a high prevalence of primary resistance to 3TC, suggesting that one important recommendation should be the realization of genotypes in all naive patients due to the high prevalence of NRTI and NNRTI mutations.