Towards high-throughput metabolomics using ultrahigh-field Fourier transform ion cyclotron resonance mass spectrometry
Towards high-throughput metabolomics using ultrahigh-field Fourier transform ion cyclotron resonance mass spectrometry
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DOI：
10.1007/s11306-008-0104-8
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发表时间：
2008-06-01
期刊：
影响因子：
3.6
通讯作者：
Borchers, Christoph H.
中科院分区：
文献类型：
--
作者：
Han, Jun;Danell, Ryan M.;Borchers, Christoph H.
傅立叶变换离子回旋共振质谱（FTICR-MS）具有无与伦比的质量分辨率、质量准确度和卓越的检测灵敏度，有可能成为高通量代谢组学分析的强大新技术。在这项研究中，我们研究了超高场12特斯拉（12 T）FTICR-MS的特性，用于人血浆代谢物的鉴定和绝对定量，以及通过直接输注（DI）对近交系小鼠血清进行非靶向代谢指纹图谱分析。使用内部质量校准（质量误差
With unmatched mass resolution, mass accuracy, and exceptional detection sensitivity, Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FTICR-MS) has the potential to be a powerful new technique for high-throughput metabolomic analysis. In this study, we examine the properties of an ultrahigh-field 12-Tesla (12T) FTICR-MS for the identification and absolute quantitation of human plasma metabolites, and for the untargeted metabolic fingerprinting of inbred-strain mouse serum by direct infusion (DI). Using internal mass calibration (mass error