Study on the pupal morphogenesis of Chrysomya rufifacies (Macquart) (Diptera: Calliphoridae) for postmortem interval estimation
Study on the pupal morphogenesis of Chrysomya rufifacies (Macquart) (Diptera: Calliphoridae) for postmortem interval estimation
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红金金蝇（Macquart）（双翅目：丽蝇科）蛹形态发生的研究，用于死后间隔估计
DOI：
10.1016/j.forsciint.2015.06.005
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发表时间：
2015-08-01
影响因子：
2.2
通讯作者：
Wang, Jiang-Feng
中科院分区：
文献类型：
--
作者：
Ma, Ting;Huang, Jia;Wang, Jiang-Feng
红腹小实蝇是我国南方地区最常见的一种死亡现场蝇类。由于其久坐不动的性质和与尸体相关联的较长持续时间，因此在死后间隔（PMI）估计中是有用的。然而，确定蛹的年龄比幼虫的年龄更困难，因为在蛹发育期间很少看到形态变化。本研究以C.在四种不同的恒定温度（分别为20、24、28和32 ℃，各+/-1 ℃）下，在具有相同饲养条件如食物、基质、光周期和相对湿度的气候室中饲养rufifacies。在不同的恒温条件下，从蛹期到羽化，每隔8h取样一次，共10个重复蛹。取蛹、处死、固定、解剖，取出蛹，观察、记录和拍照蛹的外部形态变化。形态学特征分析表明，C.描述了脓性红斑。根据28 ℃ ± 1 ℃下蛹形态发生过程中可见的外部形态特征，确定了该虫的发育历期。红层相划分为9个发育阶段，并进行了详细的描述。在上述9个发育阶段的基础上，选择了一些可见的外部形态特征作为发育阶段的标志。这些迹象映射到8小时的采样间隔在四个不同的恒温也描述了这项研究。结果表明，一般情况下，C. rufiaceae puppus与适当的发育温度呈负相关。本研究提供了比较系统的蛹发育资料。用于PMI估计的rufiphases。此外，进一步的研究工作还可以从其他环境因子、组织学分析、缩短采样间隔、PAE法和寄生虫等方面进行改进。红褐色相(C)2015爱思唯尔爱尔兰有限公司版权所有。
Chrysomya rufifacies (Macquart) is one of the most common species of blow flies at the scene of death in Southern China. Pupae are useful in postmortem interval (PMI) estimation due to their sedentary nature and longer duration of association with the corpse. However, to determine the age of a pupa is more difficult than that of a larva, due to the fact that morphological changes are rarely visible during pupal development. In this study, eggs of C. rufifacies were reared in climatic chambers under four different constant temperatures (20, 24, 28 and 32 degrees C each +/- 1 degrees C, respectively) with same rearing conditions such as foodstuff, substrate, photoperiod and relative humidity. Ten duplicate pupae were sampled at 8-h intervals from prepupae to emergence under the different constant temperatures, respectively. The pupae were sampled, killed, fixed, dissected and with the puparium removed, the external morphological changes of the pupae were observed, recorded and photographed. The morphological characters of C. rufifacies pupae were described. Based on the visible external morphological characters during pupal morphogenesis at 28 degrees C +/- 1 degrees C, the developmental period of C. rufifacies was divided into nine developmental stages and recorded in detailed description. Based on above-mentioned nine developmental stages, some visible external morphological characters were selected as indications for developmental stages. These indications mapped to 8-h sampling intervals at the four different constant temperatures were also described in this study. It is demonstrated that generally the duration of each developmental stage of C. rufifacies pupae is inversely correlated to appropriate developmental temperatures. This study provides relatively systematic pupal developmental data of C. rufifacies for the estimation of PMI. In addition, further work may improve by focus on other environmental factors, histological analysis, more thorough external examination by shortening sampling intervals, PAE (the Pupal Age Estimator) method and parasitic insects of C. rufifacies. (C) 2015 Elsevier Ireland Ltd. All rights reserved.