Acute Effects of Iron-Particle Radiation on Immunity. Part I: Population Distributions
Acute Effects of Iron-Particle Radiation on Immunity. Part I: Population Distributions
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DOI：
10.1667/rr3493.1
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
M. Pecaut;R. Dutta-Roy;Anna L. M. Smith;Tamako A. Jones;G. Nelson;D. Gridley
中科院分区：
文献类型：
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作者：
M. Pecaut;R. Dutta-Roy;Anna L. M. Smith;Tamako A. Jones;G. Nelson;D. Gridley
Pecaut，M.J.，duta-Roy，R.，Smith，A.L.，Jones，T.A.，Nelson，G.A.和Gridley，D.S.铁粒子辐射对免疫的急性影响。第一部分：人口分布。辐射。第165、68-77号决议(2006年)。暴露在高线性能量转移(LET)带电粒子中的健康风险仍不清楚。本研究的主要目的是证实和进一步表征高LET辐射(56Fe26)对全身照射后三个舱室的红细胞、血栓和白细胞的急性影响。成年雌性C57BL/6小鼠分别接受0、0.5、2、3Gy4个剂量的照射，4d后处死。用血细胞分析仪和流式细胞仪测定体重和脏器质量，并测定血、脾和骨髓白细胞。脾和胸腺(但不包括身体、肝脏和肺)质量显著减少，且呈剂量依赖关系。总体而言，红细胞(RBC)计数和大多数其他RBC参数随着剂量的增加而降低(P T>；NK和T细胞毒性>；T辅助细胞)。在骨髓中，具有粒细胞、淋巴细胞(“暗”和“亮”)和单核细胞特征的白细胞在较高的辐射剂量下表现出成比例的变化，CD34和/或Ly-6A/E的表达。这些数据与我们以前在同一动物模型中对铁离子、其他形式的辐射和太空飞行的研究相联系。
Abstract Pecaut, M. J., Dutta-Roy, R., Smith, A. L., Jones, T. A., Nelson, G. A. and Gridley, D. S. Acute Effects of Iron-Particle Radiation on Immunity. Part I: Population Distributions. Radiat. Res. 165, 68–77 (2006). Health risks due to exposure to high-linear energy transfer (LET) charged particles remain unclear. The major goal of this study was to confirm and further characterize the acute effects of high-LET radiation (56Fe26) on erythrocyte, thrombocyte and leukocyte populations in three body compartments after total-body exposure. Adult female C57BL/6 mice were irradiated with total doses of 0, 0.5, 2 and 3 Gy and killed humanely 4 days later. Body and organ masses were determined and blood, spleen and bone marrow leukocytes were evaluated using a hematology analyzer and flow cytometry. Spleen and thymus (but not body, liver and lung) masses were significantly decreased in a dose-dependent manner. In general, red blood cell (RBC) counts and most other RBC parameters were depressed with increasing dose (P T > NK and T cytotoxic > T helper cells). In the bone marrow, leukocytes with granulocytic, lymphocytic (“dim” and “bright”), and monocytic characteristics exhibited proportional variations at the higher radiation doses in the expression of CD34 and/or Ly-6A/E. The data are discussed in relation to our previous investigations with iron ions, other forms of radiation, and space flight in this same animal model.