In vivo microCT imaging of rodent cerebral vasculature
In vivo microCT imaging of rodent cerebral vasculature
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DOI：
10.1088/0031-9155/53/7/n01
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发表时间：
2008-04-07
影响因子：
3.5
通讯作者：
Hasegawa, Bruce H.
中科院分区：
文献类型：
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作者：
Seo, Youngho;Hashimoto, Tomoki;Hasegawa, Bruce H.
计算机断层扫描(CT)仍然是评估脑血管疾病患者的关键诊断工具，而专门用于为啮齿动物成像的系统的出现将这些技术扩展到这些疾病的小动物模型。因此，我们使用高分辨率紧凑型计算机断层扫描(‘microCT’)系统(FLEX(Tm)X-O(Tm)，Gamma Medica-IDEAS，加利福尼亚州北岭)对出血性中风大鼠模型的体内成像方法进行了评估。在所有活体研究中，麻醉大鼠的头部被固定在定制的固定设备中，用于MicroCT成像，在60kVp和0.530 mA(I管x t/旋转=63.6mAs)下，2min内有512个投影。首先，进行不含碘对比剂的成像(A)以区分对比剂在增强CT中的效果；(B)在CT采集的两个时间点检查固定器的有效性。然后，在持续注射碘普罗胺(300mgI·m l(-1)，1.2m l·min(-1))的情况下进行增强CT扫描，以显示蛛网膜下腔出血前颈动脉和脑动脉的动脉瘤和其他血管形成。使用FMRIB的线性图像配准工具(Flirt)，将固定装置与归一化互信息对齐的非对比和增强CT图像之间的配准的准确性进行比较。检查调情对准的非对比度CT图像和非对准的非对比度CT图像之间的平移和旋转。这两个数据集分别显示了小于0.5体素(类似于85微米)和0.5度的平移和旋转差异。平扫CT显示通过手术进入啮齿类动物大脑的股动脉血体积非常小(0.1毫升)。在CT采集过程中连续使用碘普罗胺可在重建的CT图像中产生一致的血管造影剂。结果，颈动脉和主要脑血管
Computed tomography (CT) remains a critical diagnostic tool for evaluating patients with cerebrovascular disease, and the advent of specialized systems for imaging rodents has extended these techniques to small animal models of these diseases. We therefore have evaluated in vivo methods of imaging rat models of hemorrhagic stroke using a high resolution compact computed tomography ('microCT') system ( FLEX( tm) X-O(tm), Gamma Medica-Ideas, Northridge, CA). For all in vivo studies, the head of the anesthetized rat was secured in a custom immobilization device for microCT imaging with 512 projections over 2 min at 60 kVp and 0.530 mA (I-tube x t/rotation = 63.6 mAs). First, imaging without iodinated contrast was performed ( a) to differentiate the effect of contrast agent in contrast-enhanced CT and (b) to examine the effectiveness of the immobilization device between two time points of CT acquisitions. Then, contrast-enhanced CT was performed with continuous administration of iopromide (300 mgI ml(-1) at 1.2 ml min(-1)) to visualize aneurysms and other vascular formations in the carotid and cerebral arteries that may precede subarachnoid hemorrhage. The accuracy of registration between the noncontrast and contrast-enhanced CT images with the immobilization device was compared against the images aligned with normalized mutual information using FMRIB's linear image registration tool ( FLIRT). Translations and rotations were examined between the FLIRT-aligned noncontrast CT image and the nonaligned noncontrast CT image. These two data sets demonstrated translational and rotational differences of less than 0.5 voxel (similar to 85 mu m) and 0.5 degrees, respectively. Noncontrast CT demonstrated a very small volume (0.1 ml) of femoral arterial blood introduced surgically into the rodent brain. Continuous administration of iopromide during the CT acquisition produced consistent vascular contrast in the reconstructed CT images. As a result, carotid arteries and major cerebral blood vessels were