Conducting Polymer Scaffolds for Hosting and Monitoring 3D Cell Culture
Conducting Polymer Scaffolds for Hosting and Monitoring 3D Cell Culture
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DOI：
10.1002/adbi.201700052
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发表时间：
2017-06-01
影响因子：
4.1
通讯作者：
Owens, Roisin M.
中科院分区：
文献类型：
--
作者：
Inal, Sahika;Hama, Adel;Owens, Roisin M.
这项工作报告的设计活细胞监测平台的基础上的大孔支架的导电聚合物，聚（3，4-乙撑二氧噻吩）：聚（苯乙烯磺酸）。导电聚合物支架由于其生物相容性和组织样弹性而支持3D细胞培养，这可以通过包含生物聚合物如胶原蛋白来操纵。将培养基灌注管整合在支架内使得能够在整个支架中均匀地进行细胞扩散和流体运输，从而确保长期的细胞活力。这也允许在支架内共培养多种细胞类型。在多孔结构内包含细胞影响导电聚合物网络的阻抗，因此被用作原位工具来监测细胞生长。因此，虽然导电聚合物是3D组织的组成部分，但它是一种活性成分，可增强组织功能，并形成具有集成传感能力的生物电子器件的基础。
This work reports the design of a live-cell monitoring platform based on a macroporous scaffold of a conducting polymer, poly(3,4-ethylene dioxythiophene):poly(styrenesulfonate). The conducting polymer scaffolds support 3D cell cultures due to their biocompatibility and tissue-like elasticity, which can be manipulated by inclusion of biopolymers such as collagen. Integration of a media perfusion tube inside the scaffold enables homogenous cell spreading and fluid transport throughout the scaffold, ensuring long term cell viability. This also allows for co-culture of multiple cell types inside the scaffold. The inclusion of cells within the porous architecture affects the impedance of the electrically conducting polymer network and, thus, is utilized as an in situ tool to monitor cell growth. Therefore, while being an integral part of the 3D tissue, the conducting polymer is an active component, enhancing the tissue function, and forming the basis for a bioelectronic device with integrated sensing capability.