Elevation of Microglial Basic Fibroblast Growth Factor Contributes to Development of Neuropathic Pain after Spinal Nerve Ligation in Rats
Elevation of Microglial Basic Fibroblast Growth Factor Contributes to Development of Neuropathic Pain after Spinal Nerve Ligation in Rats
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DOI：
10.1097/brs.0000000000001131
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发表时间：
2016-02-01
期刊：
影响因子：
3
通讯作者：
Takaso, Masashi
中科院分区：
文献类型：
--
作者：
Fujimaki, Hisako;Inoue, Gen;Takaso, Masashi
研究设计。采用逆转录聚合酶链反应（RT-PCR）、脊髓免疫组织学分析和大鼠神经性疼痛模型疼痛行为分析，探讨小胶质碱性成纤维细胞生长因子（bFGF）在神经性疼痛发生中的作用。目的探讨bFGF在脊髓小胶质细胞在脊髓神经结扎术后异常性疼痛发生中的作用。背景资料摘要。有证据表明，脊髓胶质细胞产生bFGF是对周围神经损伤的反应。虽然bFGF与星形胶质细胞之间的关联已被广泛报道，但bFGF与小胶质细胞之间的关系，特别是在神经性疼痛的发展方面，仍然知之甚少。术后采用RT-PCR和免疫组化检测脊髓中bFGF的表达。脊髓神经结扎后大鼠鞘内注射bFGF中和抗体。结果：脊髓结扎6 h后，大鼠脊髓中bfgf mRNA的表达明显高于未处理大鼠。免疫组织化学分析显示bFGF与离子钙结合接头分子1（一种小胶质标记物）和髓过氧化物酶共定位。结论：脊神经结扎术后，脊髓小胶质细胞中bFGF的表达增加。因此，控制周围神经损伤急性期小胶质细胞中bFGF的释放可能抑制异常性痛的进展。证据级别：无
Study Design.Reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistological analysis of spinal cord and pain behavior analysis in a rat neuropathic pain model were conducted to examine the function of microglial basic fibroblast growth factor (bFGF) in the development of neuropathic pain.Objective.To investigate the role of bFGF in spinal microglia during the development of allodynia following spinal nerve ligation in rats.Summary of Background Data.Evidence suggests that the production of bFGF by spinal cord glial cells is increased in response to peripheral nerve injury. Although an association between bFGF and astrocytes has been widely reported, the relationship between bFGF and microglia, particularly with respect to the development of neuropathic pain, remains poorly understood.Methods.Spinal nerve ligation rats were used. After surgery, bFGF expression in the spinal cord was investigated using RT-PCR and immunohistochemistry. Neutralizing antibodies to bFGF were injected intrathecally into rats after spinal nerve ligaton. Spinal cords were used for RT-PCR analysis and pain behavior was analyzed using the von Frey test.Results.bFGF mRNA expression was significantly increased in the spinal cord 6 hours after spinal nerve ligation compared with untreated rats. Immunohistochemical analysis revealed that bFGF co-localized with ionized calcium-binding adaptor molecule 1, a microglial marker, and myeloperoxidase. Neutralizing antibodies to bFGF attenuated mechanical allodynia and myeloperoxidase mRNA expression.Conclusion.bFGF increased in spinal microglia during the development allodynia after spinal nerve ligation. Thus, controlling bFGF release from microglia during the acute stage of peripheral nerve injury may suppress the progression of allodynia.Level of Evidence: N/A