Release of two Saccharomyces cerevisiae cytochrome genes, COX6 and CYC1, from glucose repression requires the SNF1 and SSN6 gene products.
Release of two Saccharomyces cerevisiae cytochrome genes, COX6 and CYC1, from glucose repression requires the SNF1 and SSN6 gene products.
复制标题
从葡萄糖抑制中释放两个酿酒酵母细胞色素基因 COX6 和 CYC1 需要 SNF1 和 SSN6 基因产物。
DOI：
10.1128/mcb.10.3.1297-1300.1990
复制
发表时间：
1990
影响因子：
5.3
通讯作者：
Poyton,RO
中科院分区：
文献类型：
--
作者：
Wright,RM;Poyton,RO
我们在这里表明，snf1和ssn6是葡萄糖抑制酵母基因cox6和cyc1的抑制所必需的，这两个基因分别编码线粒体蛋白细胞色素氧化酶亚基VI和iso1 -细胞色素。在annf1突变的遗传背景下，将细胞转移到去抑制培养基后，c0x6和cyc1的转录都继续受到抑制。在anssn6突变体遗传背景下，cox6和cyc1在抑制培养基中均高水平表达。ssn6在两个细胞色素基因的调控中上位性地作用于snf1。这些发现与之前在酿酒酵母中关于snf1和ssn6对suc2表达影响的研究结果相似，并且与提出snf1通过ssn6 oncox6和cyc1发挥作用的模型一致。
We show here thatSNF1andSSN6are required for derepression of the glucose-repressible yeast genesCOX6andCYC1,which encode the mitochondrial proteins cytochromecoxidase subunit VI and iso-1-cytochromec,respectively. In ansnf1mutant genetic background, the transcription of bothC0X6andCYC1continued to be repressed after cells were shifted into derepressing media. In anssn6mutant genetic background, bothCOX6andCYC1were expressed constitutively at high levels in repressing media.SSN6acted epistatically toSNF1in the regulation of both cytochrome genes. These findings are similar to previous findings on the effects ofSNF1andSSN6onSUC2expression inSaccharomyces cerevisiaeand are consistent with a model proposing thatSNF1exerts its effect throughSSN6onCOX6andCYC1.