Layer-by-layer assembly of nanoblended thin films: Poly(allylamine hydrochloride) and a binary mixture of a synthetic and natural polyelectrolyte
Layer-by-layer assembly of nanoblended thin films: Poly(allylamine hydrochloride) and a binary mixture of a synthetic and natural polyelectrolyte
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纳米混合薄膜的逐层组装：聚（盐酸烯丙胺）以及合成和天然聚电解质的二元混合物
DOI：
10.1021/ma0490698
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发表时间：
2004
期刊：
影响因子：
5.5
通讯作者：
F. Caruso
中科院分区：
文献类型：
--
作者：
J. Quinn;J. C. Yeo;F. Caruso
通过聚烯丙基胺盐酸盐（PAH）与单链脱氧核糖核酸（DNA）和聚苯乙烯磺酸钠（PSS）的二元混合物的层层组装形成纳米杂化多层薄膜。紫外维斯分光光度法被用作一种简便的手段来确定DNA和PSS在膜中的比例。从含有氯化钠（NaCl）的溶液中组装的膜被发现主要含有PSS，而在吸附溶液中的乙醇的存在下有利于DNA的掺入到膜中。膜的厚度通过使用表面等离子体共振来确定，其显示随着膜中DNA的比例增大而增加的膜厚度。石英晶体微天平研究证实了这一结果，其显示了增加膜中DNA百分比的较大频率增量。在高离子强度（高NaCl浓度）条件下对DNA吸附的抑制作用是由于PSS置换DNA的结果，而PSS置换DNA的结果是由于PSS的抑制作用。
Nanoblended multilayer thin films were formed by the layer-by-layer assembly of poly(allylamine hydrochloride) (PAH) and a binary mixture of single-stranded deoxyribonucleic acid (DNA) and poly(sodium styrenesulfonate) (PSS). UV−vis spectrophotometry was used as a facile means to determine the proportion of DNA and PSS in the films. Films assembled from solutions containing sodium chloride (NaCl) were found to contain predominantly PSS, while the presence of ethanol in the adsorption solution favored the incorporation of DNA into the film. The thickness of the films was determined by using surface plasmon resonance, which revealed increasing film thickness with a larger proportion of DNA in the film. This result was confirmed with quartz crystal microbalance studies, which showed a larger frequency increment for increasing DNA percentage in the film. The suppression of DNA adsorption under conditions of high ionic strength (high NaCl concentration) was shown to be a result of DNA displacement by PSS, whic...