Antibacterial Activity of Juglone against Staphylococcus aureus: From Apparent to Proteomic.
Antibacterial Activity of Juglone against Staphylococcus aureus: From Apparent to Proteomic.
复制标题
胡桃醌对金黄色葡萄球菌的抗菌活性：从表观到蛋白质组
DOI：
10.3390/ijms17060965
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发表时间：
2016-06-18
影响因子：
5.6
通讯作者：
Wu Z
中科院分区：
文献类型：
--
作者：
Wang J;Cheng Y;Wu R;Jiang D;Bai B;Tan D;Yan T;Sun X;Zhang Q;Wu Z
由病原微生物引起的食源性疾病在全球范围内的比例正在上升，葡萄球菌食物中毒是导致这一比例上升的主要原因之一。胡桃醌是一种植物来源的1，4-萘醌类化合物，具有一定的抗菌和抗肿瘤活性。然而，其抗金黄色葡萄球菌活性的具体机制尚不清楚。为了阐明其抗菌作用的机制，应用定量蛋白质组学的相对和绝对定量等压标记方法对胡桃仁处理后差异表达的53个蛋白质进行了分析。结合验证实验，如检测DNA和RNA含量的变化和氧化损伤的量化，我们的结果表明，胡桃仁有效地增加了氧化还原酶的蛋白表达，并在细胞内创造了过氧化环境，显著减少了细胞壁的形成，增加了膜的通透性。我们假设胡桃酮与DNA结合，直接减少DNA的转录和复制。这是第一次采用蛋白质组学方法来研究胡桃酮的抗菌机制。
The proportion of foodborne disease caused by pathogenic microorganisms is rising worldwide, with staphylococcal food poisoning being one of the main causes of this increase. Juglone is a plant-derived 1,4-naphthoquinone with confirmed antibacterial and antitumor activities. However, the specific mechanism underlying its antibacterial activity against Staphylococcus aureus remains unclear. To elucidate the mechanism underlying its antibacterial activity, isobaric tags for relative and absolute quantitation methods of quantitative proteomics were applied for analysis of the 53 proteins that were differentially expressed after treatment with juglone. Combined with verification experiments, such as detection of changes in DNA and RNA content and quantification of oxidative damage, our results suggested that juglone effectively increased the protein expression of oxidoreductase and created a peroxidative environment within the cell, significantly reducing cell wall formation and increasing membrane permeability. We hypothesize that juglone binds to DNA and reduces DNA transcription and replication directly. This is the first study to adopt a proteomic approach to investigate the antibacterial mechanism of juglone.