TRANSGENESIS BY MEANS OF BLASTOCYST-DERIVED EMBRYONIC STEM-CELL LINES
TRANSGENESIS BY MEANS OF BLASTOCYST-DERIVED EMBRYONIC STEM-CELL LINES
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DOI：
10.1073/pnas.83.23.9065
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发表时间：
1986-12-01
影响因子：
11.1
通讯作者：
KEMLER, R
中科院分区：
文献类型：
--
作者：
GOSSLER, A;DOETSCHMAN, T;KEMLER, R
本研究表明，囊胚来源的胚胎干细胞（ES细胞）可以作为转基因的载体。该方法几乎与其他方法一样有效，并且引入的新霉素磷酸转移酶（neomycin phosphotransferase, neo）基因可以稳定地遗传几代，而对G418的抗性没有明显损失。促进这一过程效率的一个重要因素是，在囊胚注射之前，严格选择基因转化细胞用于体外发育多能性。胚胎干细胞转基因途径的优点之一是，它为研究人员提供了在将胚胎干细胞重新引入动物之前筛选所需遗传改变的机会。
This study demonstrates that blastocyst-derived embryonic stem cells (ES cells) can be used as a vehicle for transgenesis. The method is nearly as efficient as other methods, and the introduced neomycin phosphotransferase (neo) gene is stably transmitted through several generations with no apparent loss in G418 resistance. An important factor contributing to the efficiency of this process is the rigorous selection, before blastocyst injection, of genetically transformed cells for in vitro developmental pluripotency. One of the advantages of the ES cell route to transgenesis is that it provides investigators with the opportunity to screen for the desired genetic alterations before reintroducing the ES cells into the animal.