A novel zinc finger protein is associated with U7 snRNP an interacts with the stem-loop binding protein in the histone pre-mRNP to stimulate 3′-end processing
A novel zinc finger protein is associated with U7 snRNP an interacts with the stem-loop binding protein in the histone pre-mRNP to stimulate 3′-end processing
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DOI：
10.1101/gad.932302
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发表时间：
2002-01-01
影响因子：
10.5
通讯作者：
Marzluff, WF
中科院分区：
文献类型：
--
作者：
Dominski, Z;Erkmann, JA;Marzluff, WF
茎环结合蛋白（SLBP）是后生动物细胞中组蛋白mRNA的转录后调节因子。SLBP结合组蛋白pre-mRNA，通过促进U7 snRNP与pre-mRNA的稳定结合，促进3'端加工。为了确定参与组蛋白前mrna加工的其他因素，我们使用了一种改良的酵母双杂交实验，其中SLBP及其RNA靶标共表达作为诱饵。克隆了一种新的锌指蛋白hZFP100，该蛋白与SLBP/RNA复合物相互作用，但不与游离SLBP相互作用。这种相互作用需要SLBP中对组蛋白前mrna加工很重要的区域。hZFP100抗体沉淀U7 snRNA，在爪蟾卵母细胞中表达hZFP100刺激组蛋白前体mrna的加工，表明hZFP100是加工机制的一个组成部分。
The stem-loop binding protein (SLBP) is the posttranscriptional regulator of histone mRNA in metazoan cells. SLBP binds histone pre-mRNAs and facilitates 3'-end processing by promoting stable association of U7 snRNP with the pre-mRNA. To identify other factors involved in histone pre-mRNA processing, we used a modified yeast two-hybrid assay in which SLBP and its RNA target were coexpressed as bait. A novel zinc finger protein, hZFP100, which interacts with the SLBP/RNA complex but not with free SLBP, was cloned. The interaction requires regions of SLBP that are important for histone pre-mRNA processing. Antibodies to hZFP100 precipitate U7 snRNA, and expression of hZFP100 in Xenopus oocytes stimulates processing of histone pre-mRNA, showing that hZFP100 is a component of the processing machinery.