BDCA-2, BDCA-3 and BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood
BDCA-2, BDCA-3 and BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood
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DOI：
10.4049/jimmunol.165.11.6037
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发表时间：
2000-12-01
影响因子：
4.4
通讯作者：
Schmitz, J
中科院分区：
文献类型：
--
作者：
Dzionek, A;Fuchs, A;Schmitz, J
我们已经产生了一组单克隆抗体，识别三种可能是新的人树突状细胞抗原：BDCA-2，BDCA-3和BDCA-4。在血液中，BDCA-2和BDCA-4在CD 11 c（-）CD 123（亮）浆细胞样树突状细胞上表达，而BDCA-3在少量CD 11 c（+）CD 123（-）树突状细胞上表达。所有三种Ag在第三血液树突状细胞群体上均不可检测，所述第三血液树突状细胞群体为CD 1c（+）CD 11 c（亮）CD 123（暗），或者在血液中的任何其它细胞上，BDCA-4也在单核细胞衍生的树突状细胞和CD 34（+）细胞衍生的树突状细胞上表达。一旦血液树突状细胞经历体外成熟，所有三种Ag的表达显著改变。BDCA-2在浆细胞样CD 11 c（-）CD 123（bright）树突状细胞上完全下调，BDCA-3的表达在浆细胞样CD 11 c（-）CD 123（bright）树突状细胞和CD 1c（+）CD 11 c（bright）CD 123（dim）树突状细胞上上调，BDCA-4的表达在CD 1c（+）CD 11 c（bright）CD 123（dim）树突状细胞上上调。BDCA-2在37 ℃被mAb标记后迅速内化。这三种新的抗原可作为新鲜血液中树突状细胞亚群的特异性标记物，对进一步分析和评价其治疗潜力具有重要价值。
We have generated a panel-of mAbs that identify three presumably novel human dendritic cell Ags: BDCA-2, BDCA-3, and BDCA-4. In blood, BDCA-2 and BDCA-4 are expressed on CD11c(-) CD123(bright) plasmacytoid dendritic cells, whereas BDCA-3 is expressed on small population of CD11c(+) CD123(-) dendritic cells. All three Ags are not detectable on a third blood dendritic cell population, which is CD1c(+) CD11c(bright) CD123(dim), Or on any other cells in blood, BDCA-4 is also expressed on monocyte-derived and CD34(+) cell-derived dendritic cells. Expression of all three Ags dramatically changes once blood dendritic cells undergo in vitro maturation. BDCA-2 is completely down-regulated on plasmacytoid CD11c(-) CD123(bright) dendritic cells, expression of BDCA-3 is up-regulated on both plasmacytoid CD11c(-) CD123(bright) dendritic cells and CD1c(+) CD11c(bright) CD123(dim) dendritic cells, and expression of BDCA-4 is up-regulated on CD1c(+) CD11c(bright) CD123(dim) dendritic cells. BDCA-2 is rapidly internalized at 37 degreesC after mAb labeling, The three presumably novel Ags serve as specific markers for the respective subpopulations of blood dendritic cells in fresh blood and will be of great value for their further analysis and tb evaluate their therapeutic potential.