Label-free electrochemical impedance biosensor to detect human interleukin-8 in serum with sub-pg/ml sensitivity.
Label-free electrochemical impedance biosensor to detect human interleukin-8 in serum with sub-pg/ml sensitivity.
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DOI：
10.1016/j.bios.2016.02.028
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发表时间：
2016-06-15
影响因子：
12.6
通讯作者：
Wälti C
中科院分区：
文献类型：
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作者：
Sharma R;Deacon SE;Nowak D;George SE;Szymonik MP;Tang AAS;Tomlinson DC;Davies AG;McPherson MJ;Wälti C
具有高灵敏度和短时间到结果的生物传感器能够检测体液如血清中的生物标志物，是现代医疗保健提供中早期诊断的重要先决条件。在这里，我们报告的发展电化学阻抗为基础的传感器检测血清中的人白细胞介素-8（IL-8），促血管生成的趋化因子牵连在广泛的炎症性疾病。该传感器采用基于半胱氨酸蛋白酶抑制剂支架的小而稳健的合成非抗体捕获蛋白，其对人IL-8显示出高亲和力，KD为35±10 nM，并具有出色的配体特异性。使用在0.1 Hz下测量的电化学阻抗相对于血清基线的相位变化Δ θ（Δ θ）作为定量液体中IL-8浓度的量度。在孵育15分钟后观察到最佳传感器信号，并且传感器对IL-8浓度从900 fg/ml至900 ng/ml的对数表现出线性响应。观察到的检测限约为90 fg/ml，显著低于5-10 pg/ml的基础临床水平。我们的研究结果对于发展高灵敏度和短时间的结果至关重要的护理点和早期诊断具有重要意义。研制了一种用于检测人血清中白细胞介素8（IL-8）的无标记电化学阻抗传感器。该传感器的检测限为90 fg/ml，检测时间为15 min，传感器的动态响应范围较宽，在900 fg/ml ~ 900 ng/ml范围内，传感器的响应与IL-8浓度的对数呈线性关系。该传感器采用了一种小而强大的合成非抗体捕获蛋白，具有高稳定性和出色的配体特异性。这些发现对于开发高灵敏度和短时间内获得结果至关重要的即时和早期诊断传感器特别重要。
Biosensors with high sensitivity and short time-to-result that are capable of detecting biomarkers in body fluids such as serum are an important prerequisite for early diagnostics in modern healthcare provision. Here, we report the development of an electrochemical impedance-based sensor for the detection in serum of human interleukin-8 (IL-8), a pro-angiogenic chemokine implicated in a wide range of inflammatory diseases. The sensor employs a small and robust synthetic non-antibody capture protein based on a cystatin scaffold that displays high affinity for human IL-8 with a KD of 35±10 nM and excellent ligand specificity. The change in the phase of the electrochemical impedance from the serum baseline, ∆θ(ƒ), measured at 0.1 Hz, was used as the measure for quantifying IL-8 concentration in the fluid. Optimal sensor signal was observed after 15 min incubation, and the sensor exhibited a linear response versus logarithm of IL-8 concentration from 900 fg/ml to 900 ng/ml. A detection limit of around 90 fg/ml, which is significantly lower than the basal clinical levels of 5–10 pg/ml, was observed. Our results are significant for the development of point-of-care and early diagnostics where high sensitivity and short time-to-results are essential. A label-free electrochemical impedance-based sensor for the detection of human interleukin-8 (IL-8) in full serum was developed. Detection limit of 90 fg/ml and time-to-result of 15 min was found. A large dynamic range of the sensor was observed, with sensor response linear vs logarithm of IL-8 concentration from 900 fg/ml to 900 ng/ml. The sensor employs a small and robust synthetic non-antibody capture protein, with high stability and excellent ligand specificity. Findings are particularly relevant for the development of point-of-care and early diagnosis sensors where high sensitivity and short time-to-results are essential.