Radiation response of proliferating and quiescent subpopulations isolated from multicellular spheroids.
Radiation response of proliferating and quiescent subpopulations isolated from multicellular spheroids.
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从多细胞球体中分离出的增殖和静止亚群的辐射反应。
DOI：
10.1038/bjc.1986.148
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发表时间：
1986
影响因子：
8.8
通讯作者：
Sutherland,RM
中科院分区：
文献类型：
--
作者：
Luk,CK;Keng,PC;Sutherland,RM
使用离心淘析法从EMT 6/Ro球状体中分离出两个富含G1样DNA含量的细胞亚群。两步吖啶橙子染色，然后流式细胞术，和连续的[3 H]-胸苷标记的技术同意定性，这些亚群之一主要由增殖的G1细胞，而其他包含约四倍的静止G 0/G1细胞。这两个亚群具有相似的中值细胞体积和DNA含量，但细胞体积分布不同。克隆形成是更大的“增殖”亚群比“静止”亚群。当接种的细胞数量进行了活力校正，再生研究表明，有一个更长的时间（25小时）的“静止”亚群比“增殖”亚群（10小时）之前，观察到细胞数量的任何增加。此外，相对于“增殖”细胞，“静止”细胞暴露于137 Cs γ射线辐射时更敏感。两个亚群之间的D 0相似（“增殖”G1和“静止”G 0/G1亚群的D 0分别为1.6戈伊和1.8戈伊），但“静止”亚群的辐射存活曲线肩宽减小（Dq= 2.3戈伊vs. 5.3戈伊）。
Two subpopulations enriched in cells with a G1-like content of DNA were isolated from EMT6/Ro spheroids using centrifugal elutriation. The techniques of two-step acridine orange staining followed by flow cytometry, and continuous [3H]-thymidine labelling agreed qualitatively that one of these subpopulations predominantly consisted of proliferating G1 cells, while the other contained about four times more quiescent G0/G1 cells. These two subpopulations had similar median cell volumes and DNA contents, but the cell volume distributions were different. The clonogenicity was greater in the'proliferating'subpopulation than the'quiescent'subpopulation. When cell number seeded was corrected for viability, regrowth studies showed that there was a longer time (25 h) for the'quiescent'subpopulation than the'proliferating'subpopulation (10 h) before any increase in cell number was observed. In addition, relative to the'proliferating'cells, the'quiescent'cells were more sensitive when exposed to 137Cs gamma-ray radiation. The D0's were similar between the two subpopulations (D0= 1.6 Gy and 1.8 Gy for the'proliferating'G1 and'quiescent'G0/G1 subpopulation, respectively), but the width of the shoulder of the radiation survival curve was reduced in the'quiescent'subpopulation (Dq= 2.3 Gy vs. 5.3 Gy).