Survival of human periodontal ligament cells in media proposed for transport of avulsed teeth
Survival of human periodontal ligament cells in media proposed for transport of avulsed teeth
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DOI：
10.1111/j.1600-4469.2004.00219.x
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发表时间：
2004-02-01
影响因子：
2.5
通讯作者：
Opperman, LA
中科院分区：
文献类型：
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作者：
Sigalas, E;Regan, JD;Opperman, LA
许多解决方案已经被作为撕脱牙齿的可能存储介质进行了研究。在这份报告中，人牙周韧带(PDL)细胞暴露在培养液、牛奶、Hanks平衡盐溶液(HBSS)、Soft Wear、Opti Free和Solo Care隐形眼镜溶液、佳得乐(R)和自来水中1h，在室温和冰冻条件下。用台盼蓝拒染法计数处理后即刻(0h)、24、48h的活细胞数，检测细胞的增殖能力。结果表明，当暴露在0℃时，细胞存活和增殖的数量显著增加。水对细胞有不利影响，而培养液和HBSS保存的活细胞明显多于其他实验溶液。在这项研究的参数范围内，HBSS似乎是撕脱牙的最佳储存介质。如果有冰的话，低脂牛奶可以作为替代品。如果其他溶液不容易获得，隐形眼镜溶液或冰上佳得乐((R))可以作为短期(1小时)存储介质。
Many solutions have been examined as possible storage media for avulsed teeth. In this report, human periodontal ligament (PDL) cells were exposed for 1 h to culture medium, milk, Hanks Balanced Salt Solution (HBSS), Soft Wear, Opti Free, and Solo Care contact lens solutions, Gatorade(R), and tap water, at room temperature and on ice. The number of viable cells was counted using the trypan blue exclusion technique, immediately after exposure (0 h) and at 24 and 48 h, to test the proliferative capacity of the cells after treatment. The results indicated that a significantly higher number of cells survived and proliferated when the exposures were performed at 0degreesC. Water had a detrimental effect on the cells, whereas culture medium and HBSS preserved significantly more viable cells than the other experimental solutions. Within the parameters of this study, it appears that HBSS is the optimal storage medium for avulsed teeth. Low-fat milk could serve as an alternative if ice is available. Contact lens solutions or Gatorade((R)) on ice could serve as short-term (1 h) storage media if the other solutions are not readily available.