Abnormal distribution of peripheral lymphocyte subsets induced by PDAC modulates overall survival
Abnormal distribution of peripheral lymphocyte subsets induced by PDAC modulates overall survival
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PDAC 诱导的外周淋巴细胞亚群异常分布调节总生存期
DOI：
10.1016/j.pan.2014.05.797
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发表时间：
2014-07-01
期刊：
影响因子：
3.6
通讯作者：
Yu, Xianjun
中科院分区：
文献类型：
--
作者：
Xu, Yong-Feng;Lu, Yu;Yu, Xianjun
背景/目标：免疫系统受损在恶性肿瘤患者中普遍存在，包括胰腺导管腺癌（PDAC）。本研究旨在评估PDAC患者外周血淋巴细胞亚群的变化，并评估观察到的变化的预后价值。方法：我们招募了160例连续的PDAC患者，他们在2010年和2013年之间接受了根治性手术切除。采用流式细胞术检测PDAC患者外周血淋巴细胞亚群，包括T细胞（CD 3（+）、CD 3（+）CD 4（+）、CD 3（+）CD 8（+）、CD 8（+）CD 28（+））、调节性T细胞（TcR，CD 4（+）CD 25（+）CD 127（-））、自然杀伤细胞（NK细胞，CD 3（-）CD 56（+））和B细胞（CD 19（+）），以探讨PDAC患者的预后因素。我们还评估了这些患者的临床和病理特征。结果：PDAC患者外周血淋巴细胞亚群发生了显著变化。单因素和多因素分析表明，外周血淋巴细胞亚群（CD 19（+）B细胞、TcB和CD 8（+）CD 28（+）T细胞）水平是影响总生存率的独立因素。结论：PDAC引起的机体免疫功能损害可能与患者的生存有关。版权所有（C）2014，IAP和EPC。由爱思唯尔印度出版，里德爱思唯尔印度私人有限公司的一个部门。保留所有权利。
Background/objectives: The impairment of the immune system is prevalent in patients with malignancies, including pancreatic ductal adenocarcinoma (PDAC). The present study aimed to evaluate alternations of peripheral lymphocyte subsets in patients with PDAC, and also to assess the prognostic value of observed changes.Methods: We recruited 160 consecutive PDAC patients who had undergone radical surgical resection between 2010 and 2013. To investigate the prognostic factors, we detected the peripheral lymphocyte subsets in PDAC by flow cytometry, including T cells (CD3(+), CD3(+)CD4(+), CD3(+)CD8(+), CD8(+)CD28(+)), regulatory T cells (Tregs, CD4(+)CD25(+)CD127(-)), natural killer cells (NK cells, CD3(-)CD56(+)) and B cells (CD19(+)). We also evaluated the clinical and pathological features of these patients. Survival analysis was performed by univariate and multivariate analyses.Results: Our results indicated the profile of peripheral lymphocyte subsets undergone profound changes in PDAC patients. Univariate and multivariate analysis indicated the levels of peripheral lymphocyte subsets (CD19(+) B cells, Tregs and CD8(+)CD28(+) T cells) were independent predictors for overall survival. The results also suggested that the systemic impairment of immune system in patients with PDAC, was reversed when primary tumor was removed.Conclusions: The present study provided some evidences that the impairment of host immunity induced by PDAC may play a role in the survival of patients. Copyright (C) 2014, IAP and EPC. Published by Elsevier India, a division of Reed Elsevier India Pvt. Ltd. All rights reserved.