Induction of MHC class I expression by the MHC class II transactivator CIITA
Induction of MHC class I expression by the MHC class II transactivator CIITA
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DOI：
10.1016/s1074-7613(00)80347-7
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发表时间：
1997-05-01
期刊：
影响因子：
32.4
通讯作者：
Ting, JPY
中科院分区：
文献类型：
--
作者：
Martin, BK;Chin, KC;Ting, JPY
使用主要组织相容性复合体（MHC）I类缺陷细胞系来证明MHC II类反式激活因子（CIITA）可以诱导MHC I类分子的表面表达。CIITA诱导MHC I类重链基因的启动子。位点α DNA元件是CIITA诱导的I类转录激活的靶点。此外，干扰素-γ（IFN-γ）诱导的MHC I类表达也需要一个完整的α位点。在CIITA诱导中有缺陷的G3 A细胞系不诱导响应于IFN γ的MHC I类抗原和启动子。CIITA的反式显性阴性形式降低I类MHC启动子功能和表面抗原表达。总的来说，这些数据表明CIITA在I类MHC基因诱导中具有作用。
Major histocompatibility complex (MHC) class I-deficient cell lines were used to demonstrate that the MHC class II transactivator (CIITA) can induce surface expression of MHC class I molecules. CIITA induces the promoter of MHC class I heavy chain genes. The site alpha DNA element is the target for CIITA-induced transactivation of class I. In addition, interferon-gamma (IFN gamma)-induced MHC class I expression also requires an intact site alpha. The G3A cell line, which is defective in CIITA induction, does not induce MHC class I antigen and promoter in response to IFN gamma. Trans-dominant-negative forms of CIITA reduce class I MHC promoter function and surface antigen expression. Collectively, these data argue that CIITA has a role in class I MHC gene induction.