CHANGES IN KERATIN GENE-EXPRESSION DURING TERMINAL DIFFERENTIATION OF THE KERATINOCYTE
CHANGES IN KERATIN GENE-EXPRESSION DURING TERMINAL DIFFERENTIATION OF THE KERATINOCYTE
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DOI：
10.1016/0092-8674(80)90094-x
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发表时间：
1980-01-01
期刊：
影响因子：
64.5
通讯作者：
GREEN, H
中科院分区：
文献类型：
--
作者：
FUCHS, E;GREEN, H
表皮内层细胞含有小角蛋白(相对分子质量46,000-58,000)；外层细胞除含有小角蛋白外，还含有大角蛋白(相对分子质量63,000-67,000)。角蛋白组成的变化发生在每个细胞的终末分化过程中，主要是由于合成的变化。培养的表皮细胞类似于表皮内层的细胞，只合成小的角蛋白。培养的细胞只对小角蛋白有可翻译的mRNA；从整个表皮中提取的mRNA可以翻译成大的和小的角蛋白。由于表皮最外层(角质层)没有合成，因此这一层的角蛋白必须提前合成。在某些情况下，它们会变小；这可能是通过在分化的最后阶段对分子进行翻译后处理而发生的。内脏复层鳞状上皮不形成典型的角质层，也不形成表皮特有的大角蛋白。它们的角蛋白与培养的角质形成细胞不同，这意味着它们已经走上了一条末端角蛋白合成的替代路线。
Cells of the inner layers of the epidermis contain small keratins (MW 46,000-58,000); the cells of the outer layers contain large keratins (MW 63,000-67,000) in addition to small ones. The changes in keratin composition that take place within each cell during the course of its terminal differentiation result largely from changes in synthesis. Cultured epidermal cells resemble cells of the inner layers of the epidermis in synthesizing only small keratins. The cultured cells possess translatable mRNA only for small keratins; mRNA extracted from whole epidermis can be translated into large and small keratins. As no synthesis takes place in the outermost layer of the epidermis (stratum corneum), the keratins of this layer must be synthesized earlier. In some cases they then become smaller; this presumably occurs by post-translational processing of the molecules during the final stages of differentiation. Stratified squamous epithelia of internal organs do not form a typical stratum corneum and do not make the large keratins characteristic of epidermis. Their keratins are different from those of cultured keratinocytes implying that they have embarked on an alternate route of terminal keratin synthesis.