Analysis of Transcriptional Profiling of Chamber-Specific Human Cardiac Myocytes Derived from Pluripotent Stem Cells
Analysis of Transcriptional Profiling of Chamber-Specific Human Cardiac Myocytes Derived from Pluripotent Stem Cells
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多能干细胞衍生的室特异性人心肌细胞的转录谱分析
DOI：
10.1007/978-1-0716-1484-6_20
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Yoshinori Yoshida
中科院分区：
文献类型：
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作者：
Antonio Lucena-Cacace;Yoshinori Yoshida
指导从多能干细胞到心肌细胞的细胞命运决定的分化方案通常实现高纯度和高质量的细胞。尽管如此，心脏的高度特化的解剖结构使得从多能性获得终末体细胞分化可能意味着非期望细胞谱系的异质性成为可能。定向心脏分化使通常报道含有不同比例的心室、心房和心室样细胞的细胞池分化。RNA测序（RNA-Seq）允许精确的转录谱分析，确保对我们的方案作为主要结果得出的细胞身份进行质量检查。在这里，我们描述了一个工作流程方法，如何调整RNA测序分析整合到R分析管道，以表征室特异性基因签名的主要心脏谱系的心肌细胞在心脏中。
Differentiation protocols to direct cell fate decision from pluripotent stem cells to cardiac myocytes normally achieve high purity and quality of cells. Nonetheless, the highly specialized anatomy of the heart enables the possibility that acquisition of terminal somatic differentiation from pluripotency might imply heterogeneity of non-desire cell lineages. Directed cardiac differentiation empowers differentiation of pool of cells commonly reported to contain different proportions of ventricular, atrial, and nodal-like cells. RNA sequencing (RNA-Seq) allows a precise transcriptional profiling, ensuring a quality checking of the cell identity our protocol has derived as a main outcome. Here we describe a workflow methodology on how to adapt RNA sequencing analysis for integration into the R analysis pipeline in order to characterize chamber-specific gene signatures of the major cardiac lineages of myocytes in the heart.