Synthesis of polyethylenimine modified Fe3O4 nanoparticles immobilized Cu2+ for highly efficient proteins adsorption
Synthesis of polyethylenimine modified Fe3O4 nanoparticles immobilized Cu2+ for highly efficient proteins adsorption
复制标题
聚乙烯亚胺修饰 Fe3O4 纳米颗粒固定 Cu2 的合成用于高效蛋白质吸附
DOI：
10.1016/j.colsurfa.2013.12.026
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发表时间：
2014-02-20
影响因子：
5.2
通讯作者：
Guo, Chen
中科院分区：
文献类型：
--
作者：
Xia, Tingting;Guan, Yueping;Guo, Chen
本文报道了以戊二醛（GA）为连接剂，用聚乙烯亚胺（PEI）对北京科技大学生物医学材料实验室提供的氨基功能化Fe 3 O 4纳米粒子进行修饰，以增加其表面伯胺基团的浓度。将制备的固定化亚氨基二乙酸（IDA）-Cu 2+基团的Fe 3 O 4-PEI纳米粒子应用于牛血红蛋白（BHb）和牛血清白蛋白（BSA）模型蛋白的吸附研究。采用透射电子显微镜（TEM）、振动样品磁强计（VSM）、粒度分析仪、衰减全反射-傅里叶变换红外光谱（ATR-FTIR）、茚三酮比色法和热重分析（TGA）对Fe 3 O 4-PEI NPs进行了表征。结果表明，Fe 3 O 4-PEI纳米粒子的平均粒径为172.6 nm，具有超顺磁性，饱和磁化强度为71.11 emu/g。在ATR-FTIR光谱中出现了PEI的特征峰。Zeta电位测量的研究表明，Fe 3 O 4-PEI NP的等电点增加到10.5。伯胺基团的茚三酮比色法分析表明，Fe 3 O 4-PEI纳米粒表面伯胺基团浓度增加至0.45 mmol/g，TGA表面伯胺基团浓度为0.53 mmol/g。这些结果表明，PEI已成功地修饰在胺功能化的Fe 3 O 4纳米粒子的表面，伯胺基团的浓度显着增加。蛋白质吸附分析表明，BHb的吸附量可达6000 mg/g左右，而BSA的吸附量仅为2000 mg/g左右，约为BHb的1/3。采用高效液相色谱法对蛋白质吸附的选择性进行了研究。结果表明，Fe 3 O 4-PEI-Cu ~（2+）纳米粒子对BHb的吸附量是BSA的2.5倍，进一步证明了Fe 3 O 4-PEI-Cu ~（2+）纳米粒子对蛋白质的特异性吸附。这些性质表明Fe 3 O 4-PEI-Cu 2 + NPs作为蛋白质吸附剂的潜力。(C)2013 Elsevier B. V.保留所有权利。
We report the modification of amine-functioned Fe3O4 nanoparticles (provided by the Laboratory of BioMedical Materials of University of Science and Technology Beijing) with polyethylenimine (PEI) using glutaraldehyde (GA) as a linker to increase the concentration of primary amine groups on the surface. The resultant Fe3O4-PEI NPs with immobilized iminodiacetic acid (IDA)-Cu2+ groups were applied to investigate the adsorption capacity and selectivity of model proteins: bovine hemoglobin (BHb) and bovine serum albumin (BSA). The Fe3O4-PEI NPs were characterized by transmission electron microscopy (TEM), vibrating sample magnetometer (VSM), particle analyzer, attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), ninhydrin colorimetry and thermogravimetric analysis (TGA). The results showed that the well dispersed Fe3O4-PEI NPs had an average size of 172.6 nm and were superparamagnetic with saturation magnetization of 71.11 emu/g. The characteristic peaks of PEI appeared in the ATR-FTIR spectra of Fe3O4-PEI NPs. The study of zeta potential measurement presented that the isoelectric points of the Fe3O4-PEI NPs increased to 10.5. Ninhydrin colorimetry analysis of primary amine groups showed that the concentration of primary amine groups on the surface of Fe3O4-PEI NPs increased to 0.45 mmol/g and that of TGA is 0.53 mmol/g. These results showed that PEI had been successfully modified on the surface of amine-functioned Fe3O4 NPs and the concentration of primary amine groups was significantly increased. The analysis of protein adsorption indicated that the adsorption capacity of BHb was up to about 6000 mg/g and that of BSA was only about 2000 mg/g, which was about one third of that of BHb. The research on the selectivity of protein adsorption was conducted by HPLC. The result showed that the adsorption capacity of BHb was 2.5 times as high as that of BSA, which further proved Fe3O4-PEI-Cu2+ NPs shows specific adsorption for proteins. These properties suggested the potential of the Fe3O4-PEI-Cu2+ NPs as protein adsorbent. (C) 2013 Elsevier B.V. All rights reserved.