Novel two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography, an excellent orthogonal system for practical analysis
Novel two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography, an excellent orthogonal system for practical analysis
复制标题
新型二维反相液相色谱/亲水相互作用色谱，实用分析的优秀正交系统
DOI：
10.1016/j.chroma.2008.08.079
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发表时间：
2008-10-24
影响因子：
4.1
通讯作者：
Liang, Xinmiao
中科院分区：
文献类型：
--
作者：
Liu, Yanming;Xue, Xingya;Liang, Xinmiao
建立了一种新型的二维反相液相色谱/亲水作用色谱（2D-RPLC/HILIC）系统，该系统引入了β-环糊精（β-CD）固定相。离线系统对中药中极性和中等极性成分的分离具有良好的性能。它不仅使我们能够分离一维色谱无法分离的组分，而且还可以更有效地检测低丰度组分。这种新系统的正交性是极好的。本文提出了一种简单的几何方法来表征二维液相色谱在复杂未知样品分析中的实际正交性。数学表征结果与实验结果吻合良好，均表明所提出的2D-RPLC/HILIC系统是分离极性和中等极性复杂样品的有力工具。该系统可能具有很大的潜力，分离肽和代谢组学化合物具有相似的极性。(C)2008 Elsevier B. V.保留所有权利。
A novel two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography (2D-RPLC/HILIC) system is developed with the introduction of the click beta-cyclodextrin (beta-CD) stationary phase. The offline system shows excellent abilities for the separation of polar and medium-polarity components in traditional Chinese medicine (TCM). It facilitates us not only to separate components that cannot be resolved by uni-dimensional chromatography, but also to achieve much more efficient detection of components with low abundance. The orthogonality of this new system is excellent. A simple geometric approach is developed to characterize the practical orthogonality of 2D-LC in the analysis of complex unknown samples. The mathematical characterization results are in good accordance with the experimental findings, and both demonstrate that the proposed 2D-RPLC/HILIC system is a powerful tool for the separation of polar and medium-polarity complex samples. This system may have a great potential for the separation of peptides and metabolomic compounds with similar polarity. (C) 2008 Elsevier B.V. All rights reserved.