Equine carpal articular cartilage fibronectin distribution associated with training, joint location and cartilage deterioration.
Equine carpal articular cartilage fibronectin distribution associated with training, joint location and cartilage deterioration.
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马腕关节软骨纤连蛋白分布与训练、关节位置和软骨退化相关。
DOI：
10.2746/042516400777611982
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发表时间：
2000
影响因子：
2.2
通讯作者：
A. Goodship
中科院分区：
文献类型：
--
作者：
R. Murray;H. C. Janicke;F. Henson;A. Goodship
马腕骨软骨损伤的过程尚未确定。在其他物种中，纤维连接蛋白在软骨细胞-基质相互作用中显得很重要，在骨关节炎中水平升高。本研究旨在(a)描述2岁纯种马腕关节中部的纤维连接蛋白免疫反应性，(b)确定地形变化，(c)比较剧烈训练（组1）和轻度训练（组2）的马，以及(d)描述早期骨关节炎的部位。组1 （n = 6）进行了为期19周的高强度跑步机训练。第二组（n = 6）进行40分钟步行至安乐死。桡关节面、中间关节面和第三关节面背侧和掌侧部位制备。采用生物素-链亲和素/过氧化物酶法进行免疫组化。Western blotting证实兔抗人纤维连接蛋白抗血清与马纤维连接蛋白的交叉反应性。结果显示：(a)纤维连接蛋白主要存在于细胞周和区域间基质位置，(b)与掌部部位相比，背侧部位具有区域性免疫反应性，(c)与2组相比，1组桡侧腕软骨背侧的浅表染色增加，(d)纤化软骨显示细胞内和局部基质免疫反应性（浅表区）增加。这些发现提示了纤维连接蛋白分布的地形和运动相关的变化，并表明马纤维连接蛋白定位于软骨退变部位，并通过局部软骨细胞释放到基质中。
Processes involved in equine carpal osteochondral injury have not been established. In other species, fibronectin appears important in chondrocyte-matrix interactions, and levels are increased in osteoarthritis. This investigation aimed to (a) describe fibronectin immunoreactivity in the middle carpal joint of 2-year-old Thoroughbreds, (b) determine topographical variations, (c) compare strenuously trained (Group 1) or gently exercised horses (Group 2) and (d) describe sites with early osteoarthritis. Group 1 (n = 6) underwent a 19 week high intensity treadmill training programme. Group 2 (n = 6) underwent 40 min walking until euthanasia. Dorsal and palmar sites on radial, intermediate and third carpal articular surfaces were prepared. Immunohistochemistry was performed using a biotin-streptavidin/peroxidase method. Cross-reactivity of rabbit antihuman fibronectin antiserum with equine fibronectin was confirmed using Western blotting. Results showed: (a) fibronectin was present primarily in pericellular and interterritorial matrix locations, (b) dorsal sites had zonal immunoreactivity compared to palmar sites, (c) Group 1 dorsal radial carpal cartilage had increased superficial staining compared to Group 2 and (d) fibrillated cartilage showed increased intracellular and local matrical immunoreactivity (superficial zone). These findings suggest topographical and exercise-related variations in fibronectin distribution, and indicate equine fibronectin is localised at sites of cartilage degeneration and released into the matrix by chondrocytes in the local area.
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纤连蛋白在犬骨关节炎关节软骨中的沉积。
影响因子：
1
作者：
Burton-Wurster,N;Lust,G
通讯作者：
Lust,G
三种抗关节炎药物对培养的犬关节软骨细胞纤连蛋白和硫酸角质素合成的影响。
影响因子：
1
作者：
Steinmeyer,J;Burton-Wurster,N;Lust,G
通讯作者：
Lust,G
两种骨关节炎动物模型的关节软骨中存在纤连蛋白。
DOI：
--
发表时间：
1986
期刊：
The Journal of rheumatology
影响因子：
--
作者：
Burton-Wurster,N;Butler,M;Harter,S;Colombo,C;Quintavalla,J;Swartzendurber,D;Arsenis,C;Lust,G
通讯作者：
Lust,G
正常关节软骨浅层和深层的纤连蛋白合成。
影响因子：
--
作者：
Hayashi,T;Abe,E;Jasin,HE
通讯作者：
Jasin,HE