Cholesterol-depleted cells that are relatively permissive for Semliki Forest virus infection.
Cholesterol-depleted cells that are relatively permissive for Semliki Forest virus infection.
复制标题
胆固醇耗尽的细胞相对容易受到塞姆利基森林病毒的感染。
DOI：
10.1006/viro.1996.0521
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Kielian,M
中科院分区：
文献类型：
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作者：
Marquardt,MT;Kielian,M
塞姆利基森林病毒(SFV)是一种被包裹的甲型病毒，通过内吞作用感染细胞，然后在低pH值触发病毒与内吞泡膜的融合。子代病毒是从细胞膜上萌发释放出来的，在体外，SFV与人工脂质体的融合是由低pH值触发的，依赖于靶标脂膜中胆固醇和鞘磷脂的存在。在组织培养中，当在胆固醇耗尽的昆虫细胞中进行检测时，SFV融合和病毒退出都是强烈依赖于胆固醇的。我们在这里描述了昆虫细胞的制备，这些细胞虽然不包含可检测到的胆固醇，但已经适应了类固醇耗尽的条件，导致了SFV感染的更多表型。尽管支持SFV感染的效率仍然低于对照含胆固醇细胞，但与未适应的含胆固醇细胞相比，适应细胞的SFV原发感染增加了45倍，后代病毒的释放增加，病毒生长动力学增强。适应的细胞也比低pH诱导的SFV与质膜融合的容许性高约85倍，这表明适应与细胞膜的变化有关。
Semliki Forest virus (SFV), an enveloped alphavirus, infects cells by endocytosis followed by low pH-triggered fusion of the virus and endocytic vesicle membranes. Progeny virus is released by budding from the cell plasma membrane.In vitro,SFV fusion with artificial liposomes is triggered by low pH and is dependent on the presence of cholesterol and sphingolipid in the target liposome membrane. In tissue culture, both SFV fusion and virus exit are strongly cholesterol-dependent when assayed in cholesterol-depleted insect cells. We here describe the preparation of insect cells that while not containing detectable amounts of cholesterol, have adapted to sterol-depleted conditions, resulting in a more permissive phenotype for SFV infection. Although still less efficient at supporting SFV infection than control cholesterol-containing cells, the adapted cells show a 45-fold increase in primary infection by SFV, increased release of progeny virus, and enhanced virus growth kinetics compared to nonadapted cholesterol-depleted cells. The adapted cells are also about 85-fold more permissive for low pH-induced fusion of SFV with the plasma membrane, suggesting that adaptation correlates with a change in the cell membrane.