Transcriptional slippage in the positive-sense RNA virus family Potyviridae.
Transcriptional slippage in the positive-sense RNA virus family Potyviridae.
复制标题
阳性RNA病毒家族波托病毒科中的转录滑动。
DOI：
10.15252/embr.201540509
复制
发表时间：
2015-08
期刊：
影响因子：
7.7
通讯作者：
Firth AE
中科院分区：
文献类型：
--
作者：
Olspert A;Chung BY;Atkins JF;Carr JP;Firth AE
马铃薯Y病毒科（Potyviridae）包括约30%的植物病毒，并在全球范围内造成重大经济损失。最近，一个小的重叠编码序列，称为pipo，被发现是保守的所有potyvirids的基因组中。PIPO表达为移码蛋白P3 N-PIPO的一部分，该蛋白对于病毒细胞间运动至关重要。然而，移码表达机制迄今仍是未知的。在这里，我们表明，转录滑移，特定的病毒RNA聚合酶，结果在一个人口的转录本与一个额外的“A”插入一个高度保守的GAAAAAA序列，从而使P3 N-PIPO的表达。芜菁花叶病毒的滑动效率为10.2%，滑动受到GAAAAAA序列突变的抑制。虽然转录滑移的利用在负义RNA病毒如埃博拉病毒、腮腺炎病毒和麻疹病毒中是众所周知的，但据我们所知，这是其广泛用于正义RNA病毒中的基因表达的首次报道。
The family Potyviridae encompasses ∼30% of plant viruses and is responsible for significant economic losses worldwide. Recently, a small overlapping coding sequence, termed pipo, was found to be conserved in the genomes of all potyvirids. PIPO is expressed as part of a frameshift protein, P3N-PIPO, which is essential for virus cell-to-cell movement. However, the frameshift expression mechanism has hitherto remained unknown. Here, we demonstrate that transcriptional slippage, specific to the viral RNA polymerase, results in a population of transcripts with an additional “A” inserted within a highly conserved GAAAAAA sequence, thus enabling expression of P3N-PIPO. The slippage efficiency is ∼2% in Turnip mosaic virus and slippage is inhibited by mutations in the GAAAAAA sequence. While utilization of transcriptional slippage is well known in negative-sense RNA viruses such as Ebola, mumps and measles, to our knowledge this is the first report of its widespread utilization for gene expression in positive-sense RNA viruses.