Comparison of junctional diversity in the neonatal and adult immunoglobulin repertoires.
Comparison of junctional diversity in the neonatal and adult immunoglobulin repertoires.
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DOI：
10.3109/08830189209055567
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发表时间：
1992-01-01
影响因子：
5
通讯作者：
Feeney, A J
中科院分区：
文献类型：
--
作者：
Feeney, A J
来自成年小鼠的免疫球蛋白（IG）的连接多样性对最终IG库的大小有显著贡献。在成人前B细胞中，编码区N区核苷酸的添加和缺失产生非常异质的CDR3序列。相反，来自胎儿和新生小鼠的IG显示非常有限的连接多样性。其原因是：（a）缺乏N区;和（B）某些连接序列占优势。这些共同的连接序列似乎都是通过靶向重排在待连接的片段末端附近形成短的序列同源性片段而发生的。有针对性的重排可能在某些Vh基因在个体发育早期的过度表达中发挥作用。新生儿中的这些非随机连接序列将可重复地产生某些IG，例如，优势T15抗PC抗体。因此，免疫系统首先产生可预测的IG序列的小库。就这些IG在长寿B细胞中表达的程度而言，这些早期IG序列可能在成人中持续存在。叠加在这个早期的剧目是一个非常多样化的成人IG剧目。
Junctional diversity in immunoglobulin (Ig) from an adult mouse contributes significantly to the size of the final Ig repertoire. In adult pre-B cells, N region addition and deletion of nucleotides form coding regions produces very heterogenous CDR3 sequences. In contrast, Ig from fetal and newborn mice show very restricted junctional diversity. The reasons for this are: (a) the lack of N regions; and (b) the predominance of certain junctional sequences. These common junctional sequences all appear to occur by targeted rearrangement to short stretches of sequence homology near the ends of the segments to be joined. Targeted rearrangement may play a role in the overexpression of certain Vh genes early in ontogeny. These non-random junctional sequences in the neonate will reproducibly create certain Ig, for example, the dominant T15 anti-PC antibodies. Thus the immune system first creates a small repertoire of predictable Ig sequences. To the extent that these Ig are expressed in long-lived B cells, these early Ig sequences may persist in the adult. Superimposed upon this early repertoire is an enormously diverse adult Ig repertoire.