A microchannel device for root hair isolation and behavior analysis
A microchannel device for root hair isolation and behavior analysis
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DOI：
10.1088/1361-6439/ac2bb0
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发表时间：
2021
影响因子：
2.3
通讯作者：
M. Horade;S. Nishimura;T. Yamawaki;M. Yashima
中科院分区：
文献类型：
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作者：
M. Horade;S. Nishimura;T. Yamawaki;M. Yashima
尽管用于细胞操作的微通道设备正在积极发展，但与用于动物细胞（包括人类细胞）的设备相比，用于植物的微通道设备的报道很少。本文介绍了利用微通道装置对拟南芥根系进行根毛分离和观察的方法。具体来说，为了便于观察，优化了通道的高度和宽度，使得只有一根拟南芥根毛可以通过。由于拟南芥是植物研究的模式生物，本研究将拟南芥用于实验，期望其应用范围在未来得到拓展。在典型的体外实验系统中，使用凝胶状培养基；然而，由于难以将其引入微通道器件微米级的狭窄空间，因此本研究采用液体介质。我们建立了一种独特的微通道器件协议，并成功地分离了通道高度和宽度分别为至少10 μm和10 - 14 μm的根毛。在微通道中证实了根毛伸长率，并定量证实了根毛伸长率的减速。在未来的工作中，我们打算基于本研究的结果，分析单个根毛的行为，并通过利用微通道设备的特性来阐明与驱避物质的相关性。本研究可为今后农用化学品的应用研究奠定基础。
Despite the active development of microchannel devices for cell manipulation, there are few reports on such devices for plants than those for animal cells, including human cells. This paper presents root-hair isolation and observation methods for Arabidopsis thaliana roots by using a microchannel device. Specifically, to facilitate observation, the height and width of the channel were optimized so that only one A. thaliana root hair could pass through. As A. thaliana is a model organism for plant research, in this study, it was used in a experiment with the expectation that its application will expand in the future. In typical in vitro experimental systems, a gel-like medium is used; however, as this is difficult to introduce into the micron-order narrow space of a microchannel device, a liquid medium was used in this study. We established a protocol unique to microchannel devices and succeeded in isolating root hairs at channel heights and widths of at least 10 μm and 10–14 μm, respectively. Root-hair elongation was confirmed in the microchannel, and the deceleration of root-hair elongation rate was confirmed quantitatively. In a future work, based on the findings of this study, we intend to analyze the behavior of individual root hairs and clarify the correlation with repellent substances by exploiting the characteristics of microchannel devices. This research can potentially serve as a basis for future applied research of agricultural chemicals.