Xenobiotic response in Drosophila melanogaster:: Sex dependence of P450 and GST gene induction
Xenobiotic response in Drosophila melanogaster:: Sex dependence of P450 and GST gene induction
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DOI：
10.1016/j.ibmb.2006.05.009
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发表时间：
2006-08-01
影响因子：
3.8
通讯作者：
Feyereisen, Rene
中科院分区：
文献类型：
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作者：
Le Goff, Gaelle;Hilliou, Frederique;Feyereisen, Rene
外源性物质（苯巴比妥和阿特拉津）对果蝇（Drosophila melanogaster）P450基因（一个通常与解毒相关的基因家族）表达的影响，通过DNA微阵列杂交进行了分析，并通过实时RT-PCR在两性成年人中进行了验证。只有一小部分的86个转录因子基因被外源性物质显著诱导。苯巴比妥对11个CYP基因和3个谷胱甘肽S-转移酶（GST）基因有显着诱导作用，阿特拉津对7个CYP基因和1个GST基因有显着诱导作用。Cyp 6d 5、Cyp 6 w1、Cyp 12 d1和蜕皮激素诱导的Cyp 6a 2均被这两种化学物质诱导。几个诱导基因（Cyp 6a 2，Cyp 6a 8，Cyp 6d 5，Cyp 12 d1）的组成型表达在男性高于女性，诱导水平在两种性别相似。因此，雌性的诱导水平始终高于雄性。雌性特异性和生殖调控的卵黄蛋白基因显着诱导苯巴比妥在男性和抑制阿特拉津在女性。我们的研究结果表明，果蝇的许多抗肿瘤基因选择性地对外源性物质作出反应，为果蝇提供了对化学不良环境的适应性反应。实验室筛选的菌株（Cyp 6a 2、Cyp 6a 8、Cyp 12 d1）中一些与杀虫剂抗性相关的P450基因的异生素诱导表明，P450基因表达的失调可能是获得抗性的一种简便方法。我们的研究还表明，外源性物质诱导的P450水平的变化可以通过干扰昆虫的生殖调控网络来影响昆虫的适应性。(c)2006爱思唯尔有限公司保留所有权利。
The effect of xenobiotics (phenobarbital and atrazine) on the expression of Drosophila melanogaster CYP genes encoding cytochromes P450, a gene family generally associated with detoxification, was analyzed by DNA microarray hybridization and verified by real-time RT-PCR in adults of both sexes. Only a small subset of the 86 CYP genes was significantly induced by the xenobiotics. Eleven CYP genes and three glutathione S-transferases (GST) genes were significantly induced by phenobarbital, seven CYP and one GST gene were induced by atrazine. Cyp6d5, Cyp6w1, Cyp12d1 and the ecdysone-inducible Cyp6a2 were induced by both chemicals. The constitutive expression of several of the inducible genes (Cyp6a2, Cyp6a8, Cyp6d5, Cyp12d1) was higher in males than in females, and the induced level similar in both sexes. Thus, the level of induction was consistently higher in females than in males. The female-specific and hormonally regulated yolk protein genes were significantly induced by phenobarbital in males and repressed by atrazine in females. Our, results suggest that the numerous CYP genes of Drosophila respond selectively to xenobiotics, providing the fly with an adaptive response to chemically adverse environments. The xenobiotic inducibility of some CYP genes previously associated with insecticide resistance in laboratory-selected strains (Cyp6a2, Cyp6a8, Cyp12d1) suggests that deregulation of P450 gene expression may be a facile way to achieve resistance. Our study also suggests that xenobiotic-induced changes in P450 levels can affect insect fitness by interfering with hormonally regulated networks. (c) 2006 Elsevier Ltd. All rights reserved.