Matrix effects in immunobiosensor determination of clenbuterol in urine and serum
Matrix effects in immunobiosensor determination of clenbuterol in urine and serum
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DOI：
10.1039/b316723b
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发表时间：
2004-01-01
期刊：
影响因子：
4.2
通讯作者：
Hellenäs, KE
中科院分区：
文献类型：
--
作者：
Johansson, MA;Hellenäs, KE
先前已经描述了针对β-激动剂和激素残留物的免疫化学抑制分析的构建。在目前的工作中，以尿液为主要模型基质，进一步优化了β-激动剂分析方法，以应用于生物样品。系统地研究了基质干扰对抗原-抗体相互作用和基质组分与传感器表面非特异性结合的影响。采用完全析因设计实验评价测定缓冲液成分的影响。此外，还考察了抗体浓度和样品稀释度对基质背景的影响。尿液中的NSB受缓冲液pH和盐浓度的影响较大，而缓冲液组成对抗原-抗体相互作用的基质干扰影响不大。超滤有效地防止了尿和血清样本中的NSB。增加抗体稀释度会降低基质背景，而样品稀释度会产生相反的影响。
The construction of immunochemical inhibition assays for beta-agonist and hormone residues have previously been described. In the present work the beta-agonist assay was further optimised for application to biological samples, using urine as the main model matrix. Matrix interferences with the antigen - antibody interaction and non-specific binding (NSB) of matrix components to the sensor surface were systematically studied. A full factorial design experiment was employed for evaluating the effects of assay buffer composition. In addition, the influence of antibody concentration and sample dilution on the matrix background was investigated. NSB from urine was highly affected by buffer pH and salt concentration, while buffer composition had little effect on matrix interferences with the antigen - antibody interaction. Ultra-filtration efficiently prevented NSB from urine and serum samples. Increased antibody dilution reduced the matrix background while sample dilution had an opposite effect.