Epstein-Barr virus infection of CR2-transfected epithelial cells reveals the presence of MHC class II on the virion.
Epstein-Barr virus infection of CR2-transfected epithelial cells reveals the presence of MHC class II on the virion.
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Epstein-Barr 病毒对 CR2 转染的上皮细胞的感染揭示了病毒颗粒上存在 MHC II 类。
作者：
P. Knox;Lawrence S. Young
eb病毒（EBV）受体CR2转染上皮细胞系易受EBV感染。在感染某些EBV菌株后，我们发现这些细胞成为MHC II类阳性。通过hla特异性单克隆抗体的免疫染色证实II类是病毒而非靶细胞来源。电镜免疫金染色证实病毒粒子表面存在MHC II类。虽然在EB病毒粒子上也发现了一些MHC I类，但其他细胞表面分子不存在。双色免疫荧光和共聚焦显微镜分析显示II类与ebv编码的结构蛋白（MA和VCA）在感染的上皮细胞中共定位。然而，EBV与MHC I类或MHC II类抗体的预孵育不能影响EBV结合或EBV感染。EB病毒粒子表面MHC的存在可能是细胞内途径的结果，通过该途径，生产病毒从细胞中退出，并可能影响EB病毒的靶细胞趋向性。
Epithelial cell lines transfected with the Epstein-Barr virus (EBV) receptor CR2 are susceptible to infection by EBV. Following infection with certain EBV strains we found that these cells became positive for MHC class II. The class II was confirmed as being of viral and not target cell origin by immunostaining with HLA-specific monoclonal antibodies. Electron microscopic immunogold staining confirmed the presence of MHC class II on the surface of the virion. While some MHC class I was also found on the EB virion, other cell surface molecules were absent. Dual color immunofluorescence and confocal microscopy analysis demonstrated colocalization of class II with EBV-encoded structural proteins (MA and VCA) in infected epithelial cells. However, preincubation of EBV with antibodies against either MHC class I or MHC class II failed to affect either EBV binding or EBV infection. The presence of MHC on the surface of the EB virion may be a consequence of the intracellular pathways through which productive virus exits from the cell and may influence the target cell tropism of EBV.