In vitro gene transfer to mammalian cells by the use of laser-induced stress waves:: effects of stress wave parameters, ambient temperature, and cell type
In vitro gene transfer to mammalian cells by the use of laser-induced stress waves:: effects of stress wave parameters, ambient temperature, and cell type
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DOI：
10.1117/1.2160407
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发表时间：
2006-01-01
影响因子：
3.5
通讯作者：
Obara, M
中科院分区：
文献类型：
--
作者：
Terakawa, M;Sato, S;Obara, M
激光介导基因转染作为一种新的靶向基因治疗方法，由于激光能量具有高度的空间可控性而受到广泛关注。我们之前在体内和体外都证明了应用纳秒脉冲激光诱导应力波（LISWs）可以转染质粒DNA。在本研究中，我们研究了体外转染效率与激光照射条件和应力波条件的关系。我们测量了用于基因转染的lisw的特性。对于NIH 3T3细胞，转染效率评价为激光能量和脉冲数的函数。我们还研究了环境温度的影响，发现环境温度在一定范围内的变化会导致NIH 3T3细胞转染效率的剧烈变化。不同类型细胞系的基因转染也被证明，其中细胞加热提高了非恶性细胞的转染效率，而加热降低了恶性细胞的转染效率。(c) 2006年光学仪器工程师学会。
Laser- mediated gene transfection has received much attention as a new method for targeted gene therapy because of the high spatial controllability of laser energy. We previously demonstrated both in vivo and in vitro that plasmid DNA can be transfected by applying nanosecond pulsed laser- induced stress waves (LISWs). In the present study, we investigated the dependence of transfection efficiency on the laser irradiation conditions and hence stress wave conditions in vitro. We measured characteristics of LISWs used for gene transfection. For NIH 3T3 cells, transfection efficiency was evaluated as functions of laser fluence and number of pulses. The effect of ambient temperature was also investigated, and it was found that change in ambient temperature in a specific range resulted in drastic change in transfection efficiency for NIH 3T3 cells. Gene transfection of different types of cell lines were also demonstrated, where cellular heating increased transfection efficiency for nonmalignant cells, while heating decreased transfection efficiency for malignant cells. (c) 2006 Society of Photo- Optical Instrumentation Engineers.