Discrimination of authentic Polyporus umbellatus and counterfeit by Fourier Transform Infrared and two dimensional infrared correlation spectroscopy
Discrimination of authentic Polyporus umbellatus and counterfeit by Fourier Transform Infrared and two dimensional infrared correlation spectroscopy
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DOI：
10.1016/j.molstruc.2019.126917
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发表时间：
2020-01-05
影响因子：
3.8
通讯作者：
Lan, Jin
中科院分区：
文献类型：
--
作者：
Chen, Xiangdong;Choong, YewKeong;Lan, Jin
伞形多孔菌（Polyporusumbellatus，PU）属于担子菌门多孔菌科（Polyporaceae）。菌核是其主要药用部位。它被认为是用于传统中药作为抗癌剂，因此需求量很大。这种蘑菇药用的一个主要问题是假冒产品的威胁。因此，假冒PU的检测和真实PU的确认是重要的。本研究通过收集野生PU和人工栽培PU，并与市场上常见的假冒品进行比较。傅里叶变换红外光谱（FTIR）和二维红外光谱（2D）定性分析方法由于保留了样品的原始来源，常被认为是绿色方法。在一维傅里叶变换红外光谱图中，可以清楚地看出假冒聚氨酯与正品聚氨酯的不同光谱模式。与正品PU相比，假冒PU的20nm光谱显示出超过70%的差异。这种快速简单的样品制备提高了PU定性分析的速度，并迅速确定了它们在原材料含量和某些化合物的活性位点方面的差异。(C)2019爱思唯尔B.V.保留所有权利。
Polyporus umbellatus(PU) belongs to the family Polyporaceae in the phylum of Basidiomycota. The sclerotia is its main medicinal parts. It is reputedly used in traditional Chinese Medicine as anti-cancer agent and consequently is in high demand. A major problem on the medicinal use of this mushroom is the menace of the counterfeit product. Therefore, the detection of counterfeit PU and the confirmation of genuine PU are important. This study was done by collecting wild PU and cultivated PU and compared with a counterfeit which is commonly found in the market. Fourier Transform Infrared (FTIR) and Two-Dimension Infrared (2DIR) for the qualitative analysis are often regarded as green methods because the original resources of the test sample are preserved. The different spectral patterns of the counterfeit PU compared with the genuine PU were clearly indicated in 1D FTIR spectrum. The 2DIR spectrum of counterfeit PU showed more than 70% dissimilarity compared to genuine PU. This rapid and simple sample preparation enhances the speed of qualitative analysis on PU and rapidly determines their differences in term of their raw material content and the active site of certain compounds. (C) 2019 Elsevier B.V. All rights reserved.