MASS-SPECTROMETRIC QUANTIFICATION OF INDOLE-3-ACETIC-ACID IN RHIZOBIUM CULTURE SUPERNATANTS - RELATION TO ROOT HAIR CURLING AND NODULE INITIATION
MASS-SPECTROMETRIC QUANTIFICATION OF INDOLE-3-ACETIC-ACID IN RHIZOBIUM CULTURE SUPERNATANTS - RELATION TO ROOT HAIR CURLING AND NODULE INITIATION
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DOI：
10.1128/aem.44.2.275-280.1982
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发表时间：
1982-01-01
影响因子：
4.4
通讯作者：
ROLFE, BG
中科院分区：
文献类型：
--
作者：
BADENOCHJONES, J;SUMMONS, RE;ROLFE, BG
IAA是明确地确定在根瘤菌菌株的培养上清，通过气相色谱-质谱。建立了一种以氘标记的吲哚乙酸为内标，准确定量细菌培养上清中吲哚乙酸的方法。相似的IAA浓度被发现在选定的根瘤菌突变体（缺陷的根瘤形成）和它们相应的亲本菌株的培养上清液中。由于一些突变体粘附在根毛上，根毛卷曲并不只是根瘤菌产生IAA的结果。
IAA was unequivocally identified in culture supernatants of Rhizobium strains by gas chromatography-mass spectrometry. A method for accurately quantitating IAA in bacterial culture supernatants, employing deuterium-labeled IAA as an internal standard, was developed. Similar IAA concentrations were found in culture supernatants of chosen Rhizobium mutants (defective in nodule formation) and their corresponding parent strains. Since some of the mutants adhere to root hairs, root hair curling is not simply a consequence of IAA production by rhizobia.