LORICRIN IMMUNOREACTIVITY IN HUMAN SKIN - LOCALIZATION TO SPECIFIC GRANULES (L-GRANULES) IN ACROSYRINGIA
LORICRIN IMMUNOREACTIVITY IN HUMAN SKIN - LOCALIZATION TO SPECIFIC GRANULES (L-GRANULES) IN ACROSYRINGIA
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DOI：
10.1007/bf00376822
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发表时间：
1993-11-01
影响因子：
3
通讯作者：
EADY, RAJ
中科院分区：
文献类型：
--
作者：
ISHIDAYAMAMOTO, A;HOHL, D;EADY, RAJ
兜甲蛋白是表皮细胞被膜的主要成分，也在人表皮的颗粒层中表达。在新生小鼠表皮中，兜甲蛋白在颗粒层中积累成小的圆形颗粒（L-颗粒），然后被掺入皮质细胞包膜中，但L-颗粒的表达尚未在人类皮肤中得到证实。在本研究中，我们使用植入后免疫电镜检查兜甲蛋白在正常人皮肤中的表达。我们观察到弥漫性兜甲蛋白染色的最上层颗粒细胞层interappendageal表皮，这是不相关的任何颗粒结构。在角质化细胞中，大部分标记位于角质化细胞包膜的内表面。相比之下，在肢端鞘的上段有小颗粒（L-颗粒），专门标记兜甲蛋白。在外导管细胞中，具有高度嗜锇电子密度的L-颗粒在细胞核和细胞质中明显。内导管细胞的L颗粒呈圆形或椭圆形，电子密度较低，比外导管的L颗粒大，仅存在于细胞质中。部分外导管L-颗粒和大部分内导管L-颗粒与聚丝蛋白免疫反应颗粒形成复合颗粒。这些结果表明，在人类interappendageal表皮，兜甲蛋白可能会迅速纳入corneal细胞包膜没有事先积累在任何类型的颗粒。相比之下，在acropeulingia兜甲蛋白积累在颗粒，可能反映了增加的合成或较慢的加工。内、外顶膜细胞L颗粒形态特征不同的意义尚待阐明。
Loricrin is a major component of the cornified cell envelope and is also expressed in the granular layer of human epidermis. In newborn mouse epidermis, loricrin accumulates in small round granules (L-granules) in the granular layer before being incorporated into the cornified cell envelope, but the expression of L-granules has not yet been demonstrated in human skin. In the present study we used postembedding immunoelectron microscopy to examine loricrin expression in normal human skin. We observed diffuse loricrin staining in the uppermost granular cell layer in interappendageal epidermis, that was not associated with any granular structures. rn the cornified cells, most of the labelling was on the inner face of cornified cell envelopes. By contrast, in the upper segment of acrosyringia there were small granules (L-granules) that specifically labelled for loricrin. In the outer ductal cells, L-granules with a highly osmiophilic electron density were apparent in the nucleus as well as in the cytoplasm. In the inner ductal cells, L-granules were round or oval, less electron-dense and larger than the outer ductal L-granules, and present only in the cytoplasm. Some of the outer ductal L-granules and most of the inner ductal L-granules formed composite granules with filaggrin immunoreactive granules. These results suggest that in human interappendageal epidermis, loricrin might be rapidly incorporated into the cornified cell envelope without prior accumulation in any type of granule. By contrast, in acrosyringia loricrin accumulates in granules, perhaps reflecting increased synthesis or slower processing. The significance of the different morphological features of the L-granules in inner and outer acrosyringial cells remains to be clarified.