Vibrio cholerae intestinal population dynamics in the suckling mouse model of infection
Vibrio cholerae intestinal population dynamics in the suckling mouse model of infection
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DOI：
10.1128/iai.67.8.3733-3739.1999
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发表时间：
1999-08-01
影响因子：
3.1
通讯作者：
Camilli, A
中科院分区：
文献类型：
--
作者：
Angelichio, MJ;Spector, J;Camilli, A
二十多年来，乳鼠一直被用作识别霍乱弧菌肠道定植因子的模型，但对胃内接种后可恢复微生物沿着胃肠道（GI）的位置知之甚少。在本研究中，我们确定了种群动力学的野生型和无毒突变衍生物的经典和F1 Tor生物型菌株在整个乳鼠胃肠道在不同的时间后，胃内接种。野生型菌株优先定殖在中间小肠与更近端段之间的急剧分界，其中可恢复的细胞少了许多倍。令人惊讶的是，还从盲肠和大肠中回收了大量稳定的活细胞群体。缺乏毒素协同调节丸（TCP-）的菌株从小肠中清除;然而，El Tor TCP-菌株几乎与野生型菌株一样定殖于盲肠和大肠。缺乏脂多糖O抗原（OA（-））的菌株在早期被有效地从小肠中清除，但随后在其余感染中显示净生长。此外，OA（-）菌株在大肠中保持了大量的种群。这些结果表明，对于El Tor生物型，乳鼠大肠的定殖既不需要TCP也不需要OA。最后，在感染后早期，野生型、TCP-和OA（-）菌株从小肠中的回收率相似，表明TCP和OA对于任一生物型菌株抵抗乳鼠胃肠道中的杀菌机制都不是必需的。
The suckling mouse has been used as a model to identify Vibrio cholerae intestinal colonization factors for over two decades, yet little is known about the location of recoverable organisms along the gastrointestinal (GI) tract following intragastric inoculation. In the present study, we determined the population dynamics of wild-type and avirulent mutant derivatives of both classical and Fl Tor biotype strains throughout the entire suckling mouse GI tract at various times after intragastric inoculation. Wild-type strains preferentially colonized the middle small bowel with a sharp demarcation between more proximal segments which had manyfold-fewer recoverable cells. Surprisingly, large and stable populations of viable cells were also recovered from the cecum and large bowel. Strains lacking toxin-coregulated pill (TCP-) were cleared from the small bowel; however, an El Tor TCP- strain colonized the cecum and large bowel almost as well as the wild-type strain. Strains lacking lipopolysaccharide O antigen (OA(-)) were efficiently cleared from the small bowel at early times but then showed net growth for the remainder of the infections. Moreover, large populations of the OA(-) strains were maintained in the large bowel. These results show that for the El Tor biotype neither TCP nor OA is required for colonization of the suckling mouse large bowel. Finally, similar percent recoveries of wild-type, TCP-, and OA(-) strains from the small bowel at an early time after infection suggest that TCP and OA are not required for strains of either biotype to resist bactericidal mechanisms in the suckling mouse GI tract.