Protein quantitation in 2-D gels using fluorescence with water Raman as an internal standard.
Protein quantitation in 2-D gels using fluorescence with water Raman as an internal standard.
复制标题
使用荧光以水拉曼作为内标在 2-D 凝胶中进行蛋白质定量。
DOI：
10.1021/pr0704579
复制
发表时间：
2008
影响因子：
4.4
通讯作者：
Ben-Amotz,Dor
中科院分区：
文献类型：
--
作者：
Loethen,YvetteL;Knudsen,GiselleM;Davis,Brandon;Gudihal,Ravindra;Davisson,VJo;Ben-Amotz,Dor
We have developed a method to enhance fluorescence quantification in two-dimensional gel electrophoresis using the inherent Raman scattering of water as an internal standard. We demonstrate this water internal standard (WIS) method using quantitative comparisons of commercially available protein standards that were either covalently tagged or passively stained with fluorescent tags. Thus, WIS opens up the possibility of enhancing intra- and intergel quantitative comparisons.
