Endothelin receptors and their cellular signal transduction mechanism in human cultured prostatic smooth muscle cells
Endothelin receptors and their cellular signal transduction mechanism in human cultured prostatic smooth muscle cells
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人培养前列腺平滑肌细胞内皮素受体及其细胞信号转导机制
DOI：
10.1038/sj.bjp.0701179
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发表时间：
1997
影响因子：
7.3
通讯作者：
K. Honda
中科院分区：
文献类型：
--
作者：
Y. Saita;Tomonobu Koizumi;H. Yazawa;T. Morita;T. Takenaka;K. Honda
内皮素 (ET) 受体及其细胞信号转导机制在人前列腺平滑肌细胞（HP 细胞）的原代培养物中进行了表征。 [125I]-ET-1和[125I]-ET-3结合研究表明HP细胞中同时存在ETA和ETB受体，且ETA与ETB受体的比例为1.4:1。通过逆转录聚合酶链反应对 ET 受体 mRNA 的分析也表明 HP 细胞表达 ETA 和 ETB 受体。 ET-1 和 ET-3 以浓度依赖性方式增加 HP 细胞中的细胞内游离 Ca2+ 浓度 ([Ca2+]i)。使用亚型选择性拮抗剂 BQ-123 和 BQ-788 表明 ETA 和 ETB 受体均与 [Ca2+]i 的增加有关。用百日咳毒素预处理细胞导致通过 ETA 和 ETB 受体介导的 [Ca2+]i 增加显着但部分减弱。然而，它们对百日咳毒素（PTX）的敏感性显着不同。总之，HP细胞具有ETA和ETB受体。此外，这两种内皮素受体亚型可能通过不同 GTP 结合蛋白的作用引起 [Ca2+]i 增加。
Endothelin (ET) receptors, and their cellular signal transduction mechanism, were characterized in a primary culture of human prostatic smooth muscle cells (HP cell). [125I]‐ET‐1 and [125I]‐ET‐3 binding studies revealed that both ETA and ETB receptors were present in the HP cells, and the ratio of ETA to ETB receptors was 1.4:1. Analysis of ET receptor mRNA by reverse transcription‐polymerase chain reaction also demonstrated that HP cells express both ETA and ETB receptors. ET‐1 and ET‐3 increased intracellular free Ca2+ concentration ([Ca2+]i) in the HP cells in a concentration‐dependent manner. Use of subtype selective antagonists BQ‐123 and BQ‐788, indicated that both ETA and ETB receptors were coupled to an increase in [Ca2+]i. Pretreatment of the cells with pertussis toxin resulted in a significant but partial attenuation of the [Ca2+]i increase mediated through the ETA and ETB receptors. However, sensitivity to pertussis toxin (PTX) was significantly different between them. In conclusion, HP cells possess ETA and ETB receptors. Further, these two endothelin receptor subtypes evoke an increase in [Ca2+]i possibly via the action of different GTP‐binding proteins.
A 型内皮素受体与成年大鼠心肌细胞多重反应的偶联。
影响因子：
3.6
作者：
Hilal-Dandan,R;Merck,DT;Lujan,JP;Brunton,LL
通讯作者：
Brunton,LL