CHARACTERIZATION AND SEQUENCE OF THE ESCHERICHIA-COLI STRESS-INDUCED PSP OPERON
CHARACTERIZATION AND SEQUENCE OF THE ESCHERICHIA-COLI STRESS-INDUCED PSP OPERON
复制标题
DOI：
10.1016/0022-2836(91)90379-k
复制
发表时间：
1991-07-05
影响因子：
5.6
通讯作者：
MODEL, P
中科院分区：
文献类型：
--
作者：
BRISSETTE, JL;WEINER, L;MODEL, P
我们描述了一种新的大肠杆菌操纵子，噬菌体休克蛋白(PSP)操纵子，它可以在高温、乙醇、渗透压休克和丝状噬菌体感染时诱导。操纵子至少包括四个基因：PspA、B、Cande。PSPA与内膜结合，具有蛋白质的七肽重复序列，可以形成卷曲的螺旋。该操纵子编码一个激活蛋白的因子，表达和缺失分析表明该蛋白是PSPC；PSPC被预测具有亮氨酸拉链，这是许多真核转录因子中存在的基序。PspE基因作为操纵子的一部分在压力下表达，但在正常条件下也从其自身的启动子转录而来。体外研究表明，PSPA和C在体内是被修饰的。Pspgene的表达不需要热休克因子σ32。在σ32突变体中pSP诱导持续时间的增加表明，热休克反应的一个或多个产物下调操纵子的表达。
We describe a newEscherichia colioperon, the phage shock protein (psp) operon, which is induced in response to heat, ethanol, osmotic shock and infection by filamentous bacteriophages. The operon includes at least four genes:pspA, B, CandE. PspA associates with the inner membrane and has the heptad repeats characteristic of proteins that can form coiled coils. The operon encodes a factor that activatespspexpression and deletion analyses indicate that this protein is PspC; PspC is predicted to possess a leucine zipper a motif present in many eukaryotic transcription factors. ThepspEgene is expressed in response to stress as part of the operon but is also transcribed from its own promoter under normal conditions.In vitrostudies suggest that PspA and C are modifiedin vivo. Expression of thepspgenes does not require the heat shock sigma factorσ32. The increased duration ofpspinduction in aσ32mutant suggests that a product (or products) of the heat shock response down-regulates expression of the operon.