Morphological and electrophysiological properties of principal neurons in the rat lateral amygdala in vitro
Morphological and electrophysiological properties of principal neurons in the rat lateral amygdala in vitro
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DOI：
10.1152/jn.2001.85.2.714
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发表时间：
2001-02-01
影响因子：
2.5
通讯作者：
Sah, P
中科院分区：
文献类型：
--
作者：
Faber, ESL;Callister, RJ;Sah, P
在这项研究中，我们利用急性脑切片的全细胞记录来表征大鼠外侧杏仁核棘主神经元的电生理和形态学特征。这些神经元在长时间电流注入下表现出一系列放电特性。反应各不相同，有的细胞表现出完全的峰值频率适应，峰值三到五次，有的细胞没有表现出适应。放电模式的差异在很大程度上可以用脉冲后超极化（AHP）的振幅来解释。完全适应峰值频率的细胞有大振幅的缓慢ahp，而强直放电的细胞有小振幅的缓慢ahp。在峰列期间，所有细胞都表现出类似的动作电位展宽。充满生物细胞素的神经元表现出一系列锥体样形态，树突复杂性不同，有刺状树突，并在明显显示顶端树突和基部树突的程度上有所不同。定量分析显示细胞形态和放电特性之间没有关联。我们得出的结论是，侧核神经元对体细胞电流注射的放电特性是由离子电导的差异分布决定的，而不是通过依赖于细胞形态的机制决定的。
In this study, we characterize the electrophysiological and morphological properties of spiny principal neurons in the rat lateral amygdala using whole cell recordings in acute brain slices. These neurons exhibited a range of firing properties in response to prolonged current injection. Responses varied from cells that showed full spike frequency adaptation, spiking three to five times, to those that showed no adaptation. The differences in firing patterns were largely explained by the amplitude of the afterhyperpolarization (AHP) that followed spike trains. Cells that showed full spike frequency adaptation had large amplitude slow AHPs, whereas cells that discharged tonically had slow AHPs of much smaller amplitude. During spike trains, all cells showed a similar broadening of their action potentials. Biocytin-filled neurons showed a range of pyramidal-like morphologies, differed in dendritic complexity, had spiny dendrites, and differed in the degree to which they clearly exhibited apical versus basal dendrites. Quantitative analysis revealed no association between cell morphology and firing properties. We conclude that the discharge properties of neurons in the lateral nucleus, in response to somatic current injections, are determined by the differential distribution of ionic conductances rather than through mechanisms that rely on cell morphology.