Detection and analysis of protein histidine phosphorylation
Detection and analysis of protein histidine phosphorylation
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DOI：
10.1007/s11010-009-0117-2
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发表时间：
2009-09-01
影响因子：
4.3
通讯作者：
Attwood, Paul V.
中科院分区：
文献类型：
--
作者：
Besant, Paul G.;Attwood, Paul V.
蛋白质组氨酸磷酸化是细菌、真菌和植物中信号系统的重要组成部分，越来越多的证据表明其在哺乳动物细胞生物学中的作用。与磷酸丝氨酸、磷酸苏氨酸和磷酸酪氨酸相比，磷酸组氨酸相对不稳定，特别是在用于分析蛋白质磷酸化的酸性条件下。近年来，发展特异性组氨酸磷酸化分析方法和组氨酸激酶活性测定方法的研究日益受到重视。最近的注意力集中在质谱法的应用，为此。本文综述了磷蛋白中磷酸组氨酸的检测和分析方法，特别强调了质谱技术的应用。
Protein histidine phosphorylation is well established as an important part of signalling systems in bacteria, fungi and plants and there is growing evidence of its role in mammalian cell biology. Compared to phosphoserine, phosphothreonine and phosphotyrosine, phosphohistidine is relatively labile, especially under the acidic conditions that were developed to analyse protein phosphorylation. In recent years, there has been an increasing impetus to develop specific methods for the analysis of histidine phosphorylation and assay of histidine kinase activity. Most recently attention has focussed on the application of mass spectrometry to this end. This review provides an overview of methods available for the detection and analysis of phosphohistidine in phosphoproteins, with particular emphasis on the application of mass spectrometric techniques.