Promotion of Bacillus subtilis subsp. inaquosorum, Bacillus subtilis subsp. spizizenii and Bacillus subtilis subsp. stercoris to species status
Promotion of Bacillus subtilis subsp. inaquosorum, Bacillus subtilis subsp. spizizenii and Bacillus subtilis subsp. stercoris to species status
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DOI：
10.1007/s10482-019-01354-9
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发表时间：
2019-11-12
影响因子：
2.6
通讯作者：
Zeigler, Daniel R.
中科院分区：
文献类型：
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作者：
Dunlap, Christopher A.;Bowman, Michael J.;Zeigler, Daniel R.
枯草芽孢杆菌目前包括四个亚种，枯草芽孢杆菌亚种。枯草杆菌、枯草芽孢杆菌亚种。枯草芽孢杆菌亚种。Spizizenii和枯草芽孢杆菌亚种。鸡尾酒。几项基于基因组比较的研究表明，这些亚种应该提升到物种地位。以前，将它们留为亚种的主要原因之一是缺乏可区分的表型。在这项研究中，我们使用比较基因组学来确定每个亚种特有的基因，并使用这些基因来引导我们找到独特的表型。结果表明，不同亚种之间的一个区别是它们产生不同的生物活性次级代谢产物。枯草杆菌亚种。Spizizenii被证明保存产生霉菌素、杆菌烯和3，3‘-新海藻糖二胺的基因。枯草杆菌亚种。InAquosorum被证明保守产生杆菌素F、风霉素和未知的PKS/NRPS簇的基因。枯草杆菌亚种。斯特氏杆菌被证明保守产生风霉素的基因和一个未知的PKS/NRPS簇。枯草杆菌亚种。研究表明，枯草杆菌可以保存产生3，3‘-新海藻糖二胺的基因。此外，我们更新了化学分类和表型，以支持它们提升到物种地位。
Bacillus subtilis currently encompasses four subspecies, Bacillus subtilis subsp. subtilis, Bacillus subtilis subsp. inaquosorum, Bacillus subtilis subsp. spizizenii and Bacillus subtilis subsp. stercoris. Several studies based on genomic comparisons have suggested these subspecies should be promoted to species status. Previously, one of the main reasons for leaving them as subspecies was the lack of distinguishing phenotypes. In this study, we used comparative genomics to determine the genes unique to each subspecies and used these to lead us to the unique phenotypes. The results show that one difference among the subspecies is they produce different bioactive secondary metabolites. B. subtilis subsp. spizizenii is shown conserve the genes to produce mycosubtilin, bacillaene and 3,3 '-neotrehalosadiamine. B. subtilis subsp. inaquosorum is shown conserve the genes to produce bacillomycin F, fengycin and an unknown PKS/NRPS cluster. B. subtilis subsp. stercoris is shown conserve the genes to produce fengycin and an unknown PKS/NRPS cluster. While B. subtilis subsp. subtilis is shown to conserve the genes to produce 3,3 '-neotrehalosadiamine. In addition, we update the chemotaxonomy and phenotyping to support their promotion to species status.