A Facile Preparation of Glass-supported Lipid Bilayers for Analyzing Molecular Dynamics
A Facile Preparation of Glass-supported Lipid Bilayers for Analyzing Molecular Dynamics
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DOI：
10.2116/analsci.30.1103
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发表时间：
2014-12-01
影响因子：
1.6
通讯作者：
Tokunaga, Makio
中科院分区：
文献类型：
--
作者：
Ito, Yuma;Sakata-Sogawa, Kumiko;Tokunaga, Makio
许多研究项目都关注活细胞的分子动力学。这项研究要求细胞附着在底物上，同时保持其表面分子的固有运动性。基于脂质双层的系统满足了这一要求，尽管目前的方法很复杂，它们的实用性受到限制。我们开发了一种简单、快速、可重复性地制备均一的玻璃支撑的脂质双层的方法。我们的方法为活细胞的生物成像和分子动力学分析提供了一种简便的手段。
Many research programs focus on the molecular dynamics of living cells. This research requires cells to be adhered to a substrate while retaining the innate motility of their surface molecules. Lipid bilayer-based systems fulfill this requirement, although current methods are complicated and their utility is limited. We developed a simple and rapid method for reproducible preparation of homogeneous glass-supported lipid bilayers. Our method provides a facile means for bioimaging and analysis of molecular dynamics in living cells.