WIDESPREAD OCCURRENCE OF INTERMEDIATE-SIZED FILAMENTS OF THE VIMENTIN-TYPE IN CULTURED-CELLS FROM DIVERSE VERTEBRATES
WIDESPREAD OCCURRENCE OF INTERMEDIATE-SIZED FILAMENTS OF THE VIMENTIN-TYPE IN CULTURED-CELLS FROM DIVERSE VERTEBRATES
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DOI：
10.1016/0014-4827(79)90418-x
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发表时间：
1979-01-01
影响因子：
3.7
通讯作者：
WEBER, K
中科院分区：
文献类型：
--
作者：
FRANKE, WW;SCHMID, E;WEBER, K
波形蛋白是脊椎动物间充质细胞骨架中的主要组成蛋白，针对波形蛋白的特异性抗体在豚鼠中产生。针对鼠和人波形蛋白的抗体有3种类型。针对鼠波形蛋白产生的前2种类型显示出与啮齿类动物的波形蛋白细丝的排他或优先反应。第三类针对鼠或人波形蛋白，与哺乳动物、鸟类和两栖动物等脊椎动物中的中等大小的细丝反应。免疫组化技术和免疫荧光显微镜显示，几乎所有的脊椎动物细胞生长在文化中含有丝的波形蛋白类型，通常存在于扩展阵列。这些免疫学研究结果表明，波形蛋白分子既包含在进化过程中保守的序列，也包含在不同脊椎动物物种中不同的区域。所研究的细胞不仅包括间充质来源的细胞，而且还包括来自上皮细胞的细胞，在上皮细胞中，现在可以证明在间期细胞和前角蛋白类型的中等大小的丝中存在大量的波形蛋白丝。培养中生长的大多数细胞可能含有波形蛋白类型的中等大小的细丝，而与它们所来源的细胞的分化状态无关。
Specific antibodies against vimentin, the major constitutive protein of intermediate-sized filaments present in cytoskeletons of mesenchymal cells of vertebrates, were raised in guinea pigs. Antibodies to murine and human vimentin are of 3 types. The first 2 types produced against murine vimentin show an exclusive or preferential reaction with vimentin filaments of rodents. The 3rd type raised against murine or human vimentin reacts with intermediate-sized filaments in vertebrates as diverse as mammals, birds and amphibia. Immunoreplica techniques and immunofluorescence microscopy show that almost all vertebrate cells growing in culture contain filaments of the vimentin type which are usually present in extended arrays. These immunological findings suggest that the vimentin molecule contains both sequences conserved during evolution and regions different in different vertebrate species. The cells studied include not only cells of mesenchymal origin, but also cells derived from epithelia, in which it is now possible to demonstrate extensive arrays of vimentin filaments in interphase cells and intermediate-sized filaments of the prekeratin type. Most cells grown in culture probably contain intermediate-sized filaments of the vimentin type, irrespective of the state of differentiation of the cells from which they are derived.